Plasmonic Glycan Arrays for Highly Sensitive Detection of Glycan-binding Proteins
用于高灵敏度检测聚糖结合蛋白的等离子聚糖阵列
基本信息
- 批准号:10081148
- 负责人:
- 金额:$ 22.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-08-01 至 2023-01-31
- 项目状态:已结题
- 来源:
- 关键词:AffinityAntibodiesBindingBinding ProteinsBiologicalBiological AssayBiological MarkersBiological ProcessBiologyBiomedical ResearchBiotechnologyCancer BiologyChargeChemistryClinicalComplexCore ProteinDetectionDevelopmentDiagnosticDiseaseDrug TargetingDyesEnvironmentEventFilmFluorescenceGenomicsGlassGlycobiologyGlycoproteinsGlycosaminoglycansGoalsGrowth FactorHealthImmobilizationImmunoassayInflammationInstinctKnowledgeLabelLectinLigandsMalignant NeoplasmsMass Spectrum AnalysisMeasuresMethodsModelingNanotechnologyNeoplasm MetastasisPhasePhotonsPlayPolysaccharidesProteinsProteoglycanProteomicsRBL2 geneReagentReproducibilityResearchRoleSamplingSignal TransductionSlideSmall Business Innovation Research GrantSurfaceSystemTechnologyTherapeuticThickTissuesTumor AngiogenesisVaccinesVariantanalytical toolangiogenesisbasecarbohydrate binding proteincarbohydrate structurecell behaviorcost effectivecyanine dye 5densitydesignfluorophorehigh throughput screeninginstrumentationnanorodnervous system disorderplasmonicsresponsesugartechnology developmenttechnology research and developmenttool
项目摘要
Project Summary
Exploring the biological information content in glycans represents one of the current
focuses of postgenomic research and technology development. Glycan-based high
throughput assays (e.g. glycan microarrays) hold great promise for the rapid analysis of
carbohydrate binding proteins (CBPs), elucidation of CBP biology, and the development of
diagnostics, vaccines, and therapeutics for a number of diseases, including cancer and
neurological disorders. As the field of glycobiology grows, the important roles that glycans
and glycoproteins play in health are becoming increasingly recognized. However, the “state-
of-the-art” glycan arrays have several instinct limitations including poor dynamic range, poor
sensitivity, and inability to easily interrogate multiple glycan-protein interactions
simultaneously in complex samples. Because protein–glycan interactions are typically of low
affinity, and many CBPs and auto-anti-glycan antibodies in clinical samples are generally in
the range of fg/mL to pg/mL, the detection sensitivity of current glycan arrays is unable to
reach the clinical threshold of many CBPs and auto-anti-glycan antibody biomarkers,
especially in the early stages of the diseases when their concentrations in clinical samples
very low. The goal of this project is to applied nanotechnology to overcome the major
limitations of poor sensitivity and dynamic ranges of current glycan microarrays. We aim to
develop and commercialize multi-plasmonic resonant photon enhanced fluorescent (MPREF)
glycan array for glycomic research. With this project, we expect to develop ready-to-use
products of: 1) the MPREF system include the components of plasmonic substrate and
plasmonic fluorophores which will be marketed as glycan array kit, 2) a validated model
MPREF glycan array containing 50+ Glycosaminoglycans.
项目摘要
探索聚糖中的生物信息内容是当前
后基因组研究和技术开发的重点。聚糖基高
通量测定(例如聚糖微阵列)对于快速分析
碳水化合物结合蛋白(CBP),CBP生物学的阐明,以及
诊断,疫苗和治疗许多疾病,包括癌症和
神经系统疾病随着糖生物学领域的发展,
和糖蛋白在健康中的作用越来越被人们所认识。然而,“国家-
最先进的”聚糖阵列具有几个固有的局限性,包括差的动态范围、差的
敏感性,以及无法轻松地询问多种聚糖-蛋白质相互作用
在复杂的样品中。因为蛋白质-聚糖相互作用通常较低
亲和力,并且临床样品中的许多CBP和自身抗聚糖抗体通常与抗体亲和力相关。
在fg/mL至pg/mL的范围内,目前的聚糖阵列的检测灵敏度无法
达到许多CBP和自身抗聚糖抗体生物标志物的临床阈值,
特别是在疾病的早期阶段,当它们在临床样品中的浓度
很低这个项目的目标是应用纳米技术来克服主要的
目前聚糖微阵列的灵敏度和动态范围差的局限性。我们的目标是
开发和商业化多等离子体共振光子增强荧光(MPREF)
用于糖组学研究的聚糖阵列。通过这个项目,我们希望开发出即用型
产品:1)MPREF系统包括等离子体基片的组件,
将作为聚糖阵列试剂盒销售的等离子体荧光团,2)经验证的模型
MPREF聚糖阵列含有50+糖胺聚糖。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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XICHUN ZHOU其他文献
XICHUN ZHOU的其他文献
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{{ truncateString('XICHUN ZHOU', 18)}}的其他基金
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