QTOF MASS SPECTROMETER: CELL MOLECULAR BIOLOGY, PROTEIN STRUCTURE

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：加州大学伯克利分校、化学学院和加州定量生物医学研究所(QB3)正在寻求资金，以购买配备电喷雾电离源的Q-TOF质谱仪。这台仪器将被安置在质谱学卓越中心，并将取代一台已有14年历史的仪器，该仪器缺乏满足研究人员需求的必要能力。新的卓越质谱学中心将取代目前化学学院的质谱学设施，那里的大多数工作人员运营的质谱仪无法满足现代生化和生物研究的需要。新中心将满足NIH资助的研究人员对最先进的仪器的迫切需求，以满足对非共价化合物的分析和对有限数量的化学和生化化合物的结构表征的日益增长的需求。该仪器将极大地提高质量分析的速度和准确度，并将使少量寡核苷酸、蛋白质、碳水化合物和生物无机复合体的准确质量分析成为可能。描述了六名教职员工的研究，他们每个人都将从内部最先进的电喷雾质谱仪中受益匪浅。这些研究人员中有五人已经建立了NIH的资金，而一人在过去成功地获得了NIH的资金。这六个群体包括130名博士后研究人员、研究生和本科生，他们将直接受益于这一设备。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The University of California, Berkeley, College of Chemistry and the California Institute for Quantitative Biomedical Research (QB3) are seeking funds to purchase a Q-TOF mass spectrometer equipped with an electrospray ionization source. This instrument will be housed in the Mass Spectrometry Center of Excellence and will replace a 14-year-old instrument that lacks the capabilities necessary to serve researchers' needs. The new Mass Spectrometry Center of Excellence will replace the current College of Chemistry Mass Spectrometry Facility, where most of the staff-run mass spectrometers fail to meet the needs of modern biochemical and biological research. The new Center will address a critical need for state-of-the-art instrumentation to meet the growing demand for analysis of noncovalent complexes and structural characterization of limited amounts of chemical and biochemical compounds from its NIH-funded researchers. The proposed instrument will dramatically improve the speed and accuracy with which mass analysis can be done, and will make possible accurate mass analysis of small amounts of oligonucleotides, proteins, carbohydrates, and bio-inorganic complexes. The research of six faculty members, each of whom would benefit greatly from an in-house, state-of-the- art electrospray mass spectrometer, is described. Five of these researchers have established NIH funding, while one has successfully secured NIH funding in the past. These six groups include a total of 130 postdoctoral researchers, graduate students, and undergraduate students who will benefit directly from this equipment.