FUNDS TO ACQUIRE A JEOL JSM-7401F FESEM: LUNG

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：本提案为JEOL JSM-7401F场发射扫描电子显微镜申请资金，该显微镜具有嵌入式数字成像，光束减速技术和高分辨率背散射电子成像。爱荷华大学有一台电子显微镜（1989年收购的日立S-4000）服务于整个校园。目前的系统无法在低加速电压下提供免疫金标记样品的图像，并且迫切需要第二个系统，因为只有一个系统严重限制了研究人员的访问。2004年2月1日至2005年1月31日，来自6个学院22个系54个实验室的87名研究人员使用了S-4000扫描电镜。54个学院实验室中有30个拥有NIH资助的研究经费，每年总额超过38,000,000.00美元。为了更好地支持这项研究，关键是要随时获得最先进的仪器。大量使用要求调查人员提前2-3周预订日立S-4000 SEM。该系统每天24小时供调查人员使用。此外，平均每学期有20名学生参加正式的显微镜研究课程。SEM短期课程和研讨会也定期提供。S-4000的维修停机时间接近25%，这是由于使用非常频繁，难以获得更换零件和缺乏经验的服务支持。新的FESEM将在爱荷华大学中央显微镜研究设施（CMRF）的监督下，位于埃克斯坦医学研究大楼的80室。经验丰富的工作人员将提供行政、维修以及高级技术援助和培训。机构承诺是强有力的，为研究助理提供39,000.00美元的工资支持，为持续的年度服务合同提供14,000.00美元，以及为JEOL JSM-7401 F升级冷冻器提供12,550美元。美国国立卫生研究院资助的五个提供研究描述的学院是S-4000扫描电镜的最大用户。Apicella将研究人类致病菌。Welsh将研究与Bardet- Biedl综合征和其他疾病相关的人类气道上皮细胞生物学。格罗斯参与了确定人类疱疹病毒感染的机制。Wilson检查Leismania受损的人巨噬细胞。Starner将研究血友病菌生物膜与囊性纤维化患者感染和炎症的关系。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal requests funds for a JEOL JSM-7401F Field Emission Scanning Electron Microscope with embedded digital imaging, beam decelerating technology and high resolution backscattered electron imaging. The University of Iowa has one SEM (a Hitachi S-4000 acquired in 1989) to serve the entire campus. The current system cannot provide images of immunogold labeled samples at low accelerating voltages and a second system is critically needed because having only one system is severely limiting access by investigators. From February 1, 2004 to January 31, 2005, 87 investigators from 54 faculty labs in 22 departments and 6 colleges used the S-4000 SEM. 30 of the 54 faculty labs have NIH funded research grants totaling over $38,000,000.00 annually. To better support this research, it is critical to have ready access to state-of-the-art instrumentation. Heavy usage requires Investigators to reserve the Hitachi S-4000 SEM 2-3 weeks in advance. The system is made available 24 hrs a day to accommodate investigators. In addition, an average of 20 students a semester enroll in formal Microscopy Research classes. SEM short courses and workshops are also offered on a regular basis. Downtime for maintenance on the S-4000 has been nearly 25% and is due to the combination of very heavy use, difficulty in obtaining replacement parts and inexperienced service support. The new FESEM would be conveniently located in room 80 of the Eckstein Medical Research Building under the supervision of The University of Iowa Central Microscopy Research Facility (CMRF). Administration, maintenance as well as high-level technical assistance and training will be provided by experienced staff. Institutional commitment is strong, providing $39,000.00 in salary support for a Research Assistant and $14,000.00 towards the annual service contract on a continuing basis as well as $12,550.00 to upgrade a cryostage to be used on the JEOL JSM-7401 F. The five NIH funded faculty providing research descriptions are the heaviest users of the S-4000 SEM. Apicella will study human pathogenic bacteria. Welsh will investigate human airway epithelia cell biology in relation to Bardet- Biedl syndrome and other diseases. Grose is involved in determining the mechanism of human herpes virus infections. Wilson examines Leismania compromised human macrophages. Starner will study the relationship of Haemophilus biofilms to infection and inflammation in Cystic Fibrosis patients.