Biacore T100

描述（由申请人提供）：本申请资金用于购买用于真实的测量分子相互作用的Biacore 1100表面等离子体共振（SPR）仪器。目前在加州大学洛杉矶分校校园内还没有类似的仪器，因此新仪器将大大提高当地SPR的能力。与校园中的T100最相似的仪器是Biacore 3000，它在时间分辨率、自动化和软件方面都有所下降。此外，Biacore 3000每周仅向外部用户开放6-8小时，这是不够的。因此，大多数主要用户一直在使用更容易获得的Biacore X，其功能甚至更低，特别是对于弱和低分子量相互作用，并且没有自动化。T100将取代Biacore X，Biacore X位于生物化学仪器设施中。设施经理的工资由UCLA化学和生物化学系提供，基本运营成本由UCLA-DOE实验室承担。除了这一基本的机构支持，加州大学洛杉矶分校生命科学系主任已承诺78，660美元用于购买延长保修，这将在很大程度上确保仪器的平稳运行至少5年。与现有的Biacore X一样，我们计划将T100提供给任何需要SPR的人。设施经理将负责日常维护和调度。如果出现严重的争议或其他维护问题，将由一个由三个主要用户组成的咨询委员会解决。Biacore X已经运行了7年，因此在SPR方面已经发展了相当多的专业知识。我们还计划为所有主要用户和其他相关方安排额外的T100特定培训。设施管理人员将通过个别课程和生物物理技术研究生课程进行继续培训。因此，T100将立即富有成效，并显着和广泛地推进加州大学洛杉矶分校校园的研究工作。公共卫生相关性：为了了解疾病的分子基础并设计有效的治疗干预措施，必须能够仔细测量生物分子如何相互作用。SPR是测量这些相互作用的极其重要的技术

DESCRIPTION (provided by applicant): This application requests funds for the purchase of a Biacore 1100 surface plasmon resonance (SPR) instrument for measuring molecular interactions in real time. There is currently no comparable instrument on the UCLA campus so the new instrument will significantly enhance the local SPR capabilities. The most similar instrument to the T100 on campus is a Biacore 3000, which is a step down in terms of time resolution, automation and software. Moreover, the Biacore 3000 is only accessible to outside users a mere 6-8 hours per week, which is not sufficient. Consequently most of the primary users have been using the more readily accessible Biacore X, which has even more reduced capabilities, particularly for weak and low molecular weight interactions and has no automation. The T100 will replace the Biacore X, which is housed in the Biochemistry Instrumentation facility. The salary of the facility manager is supplied by the Department of Chemistry and Biochemistry at UCLA, and basic operating costs are borne by the UCLA-DOE laboratory. In addition to this basic institutional support, the UCLA Dean of Life Sciences has committed $78,660 for the purchase of an extended warranty, which will largely ensure the smooth operation of the instrument for at least a 5 year period. Like the existing Biacore X, we plan to make the T100 available to anyone needing SPR in their work. The facility manager will be responsible for daily maintenance and scheduling. In the unlikely event of severe disputes or other maintenance issues, they will be resolved by an advisory committee consisting of three of the primary users. The Biacore X has been in operation for the past 7 years, so considerable expertise has developed in SPR. We also plan to arrange for additional, T100 specific training, for all the primary users and other interested parties. Continued training will be conducted by the Facility manager, both through individual sessions and through a graduate course on biophysical techniques. Consequently, the T100 will be immediately productive and significantly and broadly advance the research effort on the UCLA campus. Public Health Relevance: To understand the molecular basis of disease and to design effective therapeutic intervention, it is essential to be able to carefully measure how biological molecules interact. SPR is an extremely important technique for measuring these interactions