PROTEOMICS CORE

蛋白质组学仪器、方法和信息学的最新进展创造了令人兴奋的 在糖尿病研究的所有领域的机会。蛋白质鉴定和 质谱法提供的结构可应用于糖尿病的几乎所有方面， 并发症基于质谱的蛋白质组学加速了翻译后蛋白质的鉴定， 修饰，包括磷酸化位点作图，蛋白质-蛋白质鉴定 相互作用，以及蛋白质丰度或区室化的变化，仅举几例 例子.然而，蛋白质组学技术的快速增长和变化也导致了 这些技术的翻译和研究人员的可用性方面的挑战，从新的 博士后研究员，以建立调查谁不是直接参与这一领域。 虽然质谱法的许多基本原理相对简单， 成功的基于质谱的蛋白质组学分析需要适当的 实验设计，使用最先进的仪器，严格分析光谱数据， 对于某些研究，生物信息学工具可以管理和解释大型数据集。事实上，质量 基于光谱的蛋白质组学是一个多步骤的过程，研究设计，从以下角度来看， 质谱数据的获取和解释，在实验中起着重要的作用 成功蛋白质组学核心的总体目标是为乔斯林研究人员提供 通过蛋白质组学研究的工作流程提供帮助，包括实验设计，样品 制备、质谱分析、数据分析和解释以及生物信息学工具。 Joslin蛋白质组学核心的具体目标如下： 1)协助并提供蛋白质组学研究实验设计的培训。 2)提供常规和定制的基于质谱的蛋白质组学分析。 3)协助质谱分析、解释和数据库匹配。 4)开发一个结果数据库，并纳入生物信息学工具，用于分析 将蛋白质组学数据输入结果数据库。

The recent advances in proteomic instrumentation, methods and informatics have created exciting opportunities in all fields of diabetes research. The information on protein identification and structure provided by mass spectrometry is applicable to nearly all aspects of diabetes and its complications. Mass spectrometry-based proteomics has accelerated the identification of posttranslational modifications, including phosphorylation-site mapping, identification of protein-protein interactions, and changes in protein abundance or compartmentalization, just to name a few examples. However, the rapid rates of growth and change in proteomic technologies have also led to challenges in the translation and availability of these technologies to researchers, from new postdoctoral research fellows to established investigators who are not directly involved in this field. Although many of the fundamental principles of mass spectrometry are relatively straightforward, successful mass spectrometry-based proteomic analysis requires the combination of appropriate experimental design, access to state-of-the-art instrumentation, rigorous analysis of spectral data, and for some studies, bioinformatics tools to manage and interpret large datasets. Indeed, mass spectrometry-based proteomics is a multi-step process, and study design, from the perspectives of mass spectrometry data acquisition and interpretation, plays an important role in experimental success. The overall objective of the Proteomics Core is to provide Joslin researchers with assistance through the workflow of proteomics studies, including experimental design, sample preparation, mass spectrometric analysis, data analysis and interpretation, and bioinformatic tools. The specific objectives of Joslin's Proteomics Core are the following: 1) To assist and provide training in experimental design for proteomics studies. 2) To provide routine and custom mass spectroscopy-based proteomic analyses. 3) To assist with mass spectra analysis, interpretation, and database matching. 4) To develop a results database and incorporate access to bioinformatic tools for the analysis of proteomics data into the results database.