OPTIMIZATION OF METHODS FOR PRODUCTION OF BOTH ICSI- AND SCNT DERIVED BABOON

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。在这个方案中，狒狒将被药物人工刺激以产生卵子，这些卵子将通过微创腹部手术（腹腔镜）或超声引导下的抽吸收集。这些卵子将在实验室中用新鲜或冷冻精子受精。一旦受精，胚胎将被培养到早期细胞分裂阶段，以获得狒狒干细胞。一些卵子可能会被移植来制造克隆胚胎。这些胚胎也将被培养到早期细胞分裂阶段，以获得干细胞。我们还将收集激素刺激和定时交配后的雌性胚胎。用培养基冲洗子宫，经宫颈收集胚胎。这些胚胎也将被培养，然后用于干细胞衍生。我们期望这项研究将使我们能够优化狒狒胚胎干细胞的衍生方法。任何已建立的细胞系都将经过严格的测试，以确定其效力和正常情况。虽然人类胚胎干细胞在治疗帕金森病、糖尿病、自闭症、心脏病、阿尔茨海默病等多种疾病方面可能具有令人难以置信的治疗价值，但在开始任何此类试验之前，必须在动物模型中进行严格的测试。狒狒提供了一种有价值的非人类灵长类动物模型，用于确定免疫匹配干细胞的安全性和有效性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. For this protocol, baboons will be artificially stimulated with drugs to produce eggs that will be collected via minimally invasive abdominal surgery (laparoscopy) or ultrasound-guided aspiration. These eggs will be fertilized in the laboratory using fresh or frozen sperm. Once fertilized, the embryos will be cultured to the early cell division stage in order to derive baboon stem cells. Some eggs may undergo transfer to create cloned embryos. These embryos will also be cultured to the early cell division stage to derive stem cells. We will also collect embryos from females after hormonal stimulation and time-mating. These embryos are collected transcervically by flushing the uterus with medium. These embryos will also be cultured and then use for stem cell derivations. We anticipate that this research will enable us to optimize methods for the derivation of baboon embryonic stem cells. Any established lines will undergo rigorous testing to determine their potency and normalcy. While human embryonic stem cells may have incredible therapeutic value for treating a wide range of disorders such as Parkinson's diabetes, autism, heart disease, Alzheimer's etc., rigorous testing in animal models is necessary before any such trials can begin. Baboons provide a valuable non-human primate model in which to determine the safety and efficacy of immune-matched stem cells.