GLUCOREGULATORY PEPTIDE HORMONES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。这些研究的总体目标（R01DK002700）旨在确定脂肪酸（FFA）如何在肥胖早期刺激β细胞代偿，但在肥胖晚期导致代偿β细胞功能障碍和死亡以及NIDDM。我们也对FFA对远端组织的直接作用感兴趣；特别是储存的甘油三酯如何影响心脏功能。这项最新研究的目的是开发和验证质子磁共振波谱（1H MRS）作为一种体内工具来测量心肌脂质含量。在离体大鼠组织和15名健康人体内的补充研究证明，1H MRS是一种可重复的心肌甘油三酯测量技术。在Zucker大鼠心肌组织中，1H MRS测量的甘油三酯与生化测量的结果吻合（P < 0.001）。在人类实验对象中，即使是非常瘦的人心脏中的甘油三酯也很明显，超重和肥胖的人心脏中的甘油三酯也会升高。心肌甘油三酯含量增加，伴有左室质量升高和室间隔壁增厚抑制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The general goal of these studies (R01DK002700) are designed to determine how fatty acids (FFA) stimulate beta-cell compensation early in obesity and yet cause dysfunction and death of compensated beta-cells and NIDDM late in obesity. We are also interested in the direct effects of FFA on remote tissues; particularly how stored triglycerides impact cardiac function. The goal of this recent study was to develop and validate proton magnetic resonance spectroscopy (1H MRS) as an in vivo tool to measure myocardial lipid content. Complementary studies in rat tissue ex vivo and in 15 healthy humans in vivo provided evidence that 1H MRS constitutes a reproducible technique for the measurement of myocardial triglyceride. In myocardial tissue from Zucker rats, the 1H MRS measurement of triglyceride matched that obtained by biochemical measurement (P 0.001). In human subjects triglyceride was evident in the hearts of even the very lean individuals and was elevated in overweight and obese subjects. Increased myocardial triglyceride content was accompanied by elevated LV mass and suppressed septal wall thickening as measured by cardiac imaging.