TOWARDS A SINGLE INJECTION METHOD OF DETERMINING IN VIVO PH BY MRI

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。以非侵入性方式确定体内pH的能力将代表肿瘤学的重大进步。MRI造影剂现在被广泛使用，但目前临床使用的试剂对生物信息不敏感，例如离子浓度、酶活性或特异性结合位点的存在的变化。尽管能够报告pH值的试剂已被发现多年，但没有一个超过几个小动物成像研究。当设计通过MRI测量体内pH的系统时，所面临的最重要的问题是必须知道造影剂的浓度以便生成pH图。在这个项目中，我们将开发一个简单，方便的解决方案来解决这个问题。本研究的目标是向该系统中添加浓度标记组件，以提供单一可注射剂溶液，其将在单一成像研究中产生组织的细胞外pH的图像图。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The capability to determine in vivo pH in a non-invasive manner would represent a major advance in oncology. MRI contrast media are now widely used but the agents currently in clinical use are insensitive to biological information such as changes in ion concentrations, enzyme activity or the presence of specific binding sites. Despite the fact that agents capable of reporting pH have been known for a number of years, none have gone beyond a few small animal imaging studies. The most significant problem faced when designing a system to measure pH in vivo by MRI is that the concentration of the contrast agent must be known in order to generate a pH map. We will develop a simple, convenient solution to this problem in this project. The goal of the present research is to add a concentration marker component to this system to provide a single injectable agent solution that will yield an image map of extracellular pH of tissue in a single imaging study.