LOOKING FOR RNA CARGOS REGULATED BY FMRP

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。FMRP是由脆性X综合征突变而来的蛋白，脆性X综合征是人类常见的智力低下。FMRP也是一种RNA结合蛋白，可能调控RNA在神经元中的转运及其翻译。我们通过apra芯片鉴定了几个与FMRP相关的rna。其中之一是R1，它是单胺氧化酶a （MAO-A）的转录调节因子。现在我们试图检测野生型和FMRP敲除小鼠的MAO-A活性。我们使用的MAO底物是荧光蛋白- Kynuramine。LFD中的技术对我们获得结果是非常重要的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. FMRP is the protein mutated from fragile X syndrome, which is a common mental retardation in human. FMRP is also a RNA binding protein and may regulate RNA transport in neurons and its translation. We identified several RNAs associated with FMRP by APRA-microarray. One of them is R1, which is a transcription regulator of Monoamine oxidase-A (MAO-A). Now we are tried to detect the MAO-A activity in wild type and FMRP knockout mice. And the MAO substrate we are using is fluorescent protein- Kynuramine. The technique in LFD is very important for us to get the results.