T1-RELAXIVITIES AS FUNCTION OF PORPHYRINS AND TEMPERATURE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。该项目的目的是开发一种顺铂/加多啶温度敏感脂质体（TSL），该脂质体将允许使用MRI T1缩短来测量顺铂的脂质体浓度。本工作的第二个目标是评估几种锰卟啉的弛性，并测试它们包封在TSL中的潜力。该项目首先需要校准1/T1作为室温下存在脂质的游离gadoteridol浓度的函数。该校准将用于验证脂质体的装载和体外释放。在最终的脂质体配方中，加多特idol和顺铂都将被包裹到脂质体中，并在脂质体释放前后测量T1弛豫度。为了准备未来结合热疗的体内研究，还需要校准T1弛豫度作为温度的函数。同样的程序将适用于涉及卟啉锰溶液的体外试验，目的是确定它们的松弛度，以及用于封装它们的TSL脂质体的释放特性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This aim of this project is to develop a cisplatin/gadoteridol temperature-sensitive liposome (TSL) that will allow the use of MRI T1 shortening to measure the liposomal concentration of cisplatin. A secondary goal of this work is to evaluate the relaxivity of several manganese porphyrins, and test the potential of their encapsulation into TSL. The project first requires calibration of 1/T1 as a function of free gadoteridol concentration at room temperature in the presence of lipid. This calibration will be used to verify liposomal loading and release in vitro. In the final liposome formulation, gadoteridol and cisplatin will both be encapsulated into the liposomes, and T1 relaxivity will be measured before and after liposome release. To prepare for future in vivo studies in combination with hyperthermia, it is also necessary to calibrate T1 relaxivity as a function of temperature. The same procedure will be applied to in vitro tests that involve manganese porphyrin solutions, with the purpose of determination of their relaxivity, and release characteristics of TSL liposomes that will be used to encapsulate them.