THE STRUCTURE OF MITOCHONDRIA IN ROD AND CONE PHOTORECEPTORS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。具体目标-以前，我们的特点和比较线粒体嵴地形和连接，矩阵体积和接触网站的杆和锥内节（RIS和CIS）从野生型C57 BL/6小鼠使用电子断层扫描。由于出生后铅暴露产生杆选择性细胞凋亡，我们还研究了RIS线粒体野生型和转基因小鼠过度表达Bcl-xL的光感受器（Tg）暴露于水或铅（Pb）的解决方案在出生后的发展。今年研究的目的是类似地表征野生型杆（小球）和锥（蒂）的突触末端（ST）中的线粒体，以及在出生后发育过程中暴露于水或铅溶液的野生型和Tg小鼠的杆小球中的线粒体，然后比较各自的线粒体ST和IS结果。 出版物：约翰逊，J.E.，帕金斯，佐治亚州，布朗，J.，Lahsaei，P.，Ghassemzadeh，S.，Ellisman M.H.，Fox，D.A.（2006）.对照、铅暴露和/或Bcl-xL转基因小鼠视杆细胞突触末端线粒体的三维电子断层扫描分析。ARVO会议。2006年5月，佛罗里达州劳德代尔堡。 Perkins，Guy A.;作者：Brown，约书亚; Lahsaei，Peiman; Ghassemzadeh，Sassan; Ellisman，Mark H.;约翰逊，杰里E.;唐纳德·福克斯（2006）Bcl-Xl过表达保护杆光感受器突触末端线粒体免受铅诱导的渗透性转变。毒理学学会会议。San Diego，CA，Mar. 2006.

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific Aims - Previously we characterized and compared mitochondrial cristae topography and connectivity, matrix volume and contact sites of rod and cone inner segments (RIS & CIS) from wild-type C57BL/6 mice using electron tomography. Since postnatal lead exposure produces rod-selective apoptosis, we also examined RIS mitochondria from wild-type and transgenic mice overexpressing Bcl-xL in photoreceptors (Tg) exposed to either water or lead (Pb) solution during postnatal development. The aim of the study this year was to similarly characterize the mitochondria in synaptic terminals (ST) of wild-type rods (spherules) and cones (pedicles) and in rod spherules of wild-type and Tg mice exposed to either water or Pb solution during postnatal development and then to compare the respective mitochondrial ST and IS results. Publications: Johnson, J.E., Perkins, G.A., Brown, J., Lahsaei, P., Ghassemzadeh, S., Ellisman M.H., and Fox, D.A. (2006). A Three-Dimensional Electron Tomographic Analysis of Rod Photoreceptor Synaptic Terminal Mitochondria in Control, Lead-Exposed, and/or Bcl-xL Transgenic Mice. ARVO Meeting. Fort Lauderdale, FL. May 2006. Perkins, Guy A.; Brown, Joshua; Lahsaei, Peiman; Ghassemzadeh, Sassan; Ellisman, Mark H.; Johnson, Jerry E.; Fox, Donald A. (2006) Bcl-Xl Overexpression Protects Rod Photoreceptor Synaptic Terminal Mitochondria From Lead-Induced Permeability Transition. Society of Toxicology Meeting. San Diego, CA, Mar. 2006.