MOBILITY STUDY OF ALLYLAMINE BY FRAP

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我们感兴趣的是利用光漂白后荧光恢复(FRAP)这一基于单分子的技术来研究表面吸附的聚烯丙基胺的迁移率。这项技术让我们可以直接测量蛋白质和脂类在细胞膜中的横向转运速度。对我们来说，最感兴趣的三个问题是：(1)聚烯丙胺在任何条件下是否从[底层为聚苯乙烯磺酸]的逐层表面剥离，例如在酸、碱和高盐浓度下，(2)在任何条件下，外层的聚烯丙胺是否有任何横向迁移性；即在此外层内的FRAP，以及(3)在稳定性和横向迁移性方面，我们新型的共价交联聚烯丙胺表面与传统的离子交联逐层表面相比如何。为此，聚烯丙基胺被标记了一种荧光染料，该染料可以被514 nm的氩离子激光器激发，该激光又耦合到共聚焦显微镜。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are interested to study the mobility of surface adsorbed polyallylamine using Fluorescence Recovery After Photobleaching (FRAP), a single molecule based technique. This technique gives us a direct measure of the rates of lateral transport of proteins and lipids in cell membranes. For us, the three issues of greatest interest are: (1) Does poly(allylamine) come off of a layer-by-layer surface [with an underlayer of poly(styrenesulfonate)] under any conditions; e.g., acid, base high salt concentration, (2) is there any lateral mobility of the outer poly(allyamine) layer under any conditions; i.e., FRAP within this outer layer, and (3) how does our novel covalently-cross-linked poly(allylamine) surface compare with conventional ionically-cross-linked, layer-by-layer surfaces in terms of stability and lateral mobility. For this the polyallylamine is labeled with a fluorescent dye that can be excited by the 514 nm line of an Argon-ion laser which in turn is coupled to a confocal microscope.