MACCHESS PROGRAM FOR MICROCRYSTALLOGRAPHY/MEMBRANE PROTEIN CRYSTALS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我们刚刚开始用内介观法对膜蛋白进行衍射研究（F1线）。2003年9月，我们在这项拨款的支持下第一次访问国际象棋时进行了测量。数据采集采用最大尺寸为40微米的低温微晶。迄今为止，最好的结果是细菌视紫红质（bR）的衍射为1.8¿，光收集复合物II （Lh2）蛋白的衍射为2.8¿。用分子置换法求解了bR的结构。bR/Lh2的衍射数据统计如下：空间群：P63 / P212121；分辨率极限：40 - 1.85/50 - 2.8；独特反射：20,214/26,790；完整性（%）：96.7/98.6；我/σ:29.9/13.3;Rsym(%): 7.0/9.4。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have just begun diffraction studies (F1 line) on the membrane proteins grown in-house by the in meso method. Measurements were made during our first trip to CHESS under the auspices of this grant in September, 2003. Data were collected using cryocooled microcrystals of maximum dimension 40 micron. Best results to date are diffraction to 1.8 ¿ for bacteriorhodopsin (bR) and 2.8 ¿ for the light harvesting complex II (Lh2) protein. Structures of bR have been solved using molecular replacement. Diffraction data statistics for bR/Lh2 are as follows: Space group: P63 / P212121; Resolution limits (¿): 40 - 1.85/50 - 2.8; Unique reflections: 20,214/26,790; Completeness (%): 96.7/98.6; I/sigma: 29.9/13.3; Rsym (%): 7.0/9.4.