DIGITAL DATA ACQUISITION SYSTEM

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。DiDAC系统目前在四台LANL流式细胞仪（两台相敏流式细胞仪，三激光系统和快速混合动力学流式细胞仪）上常规使用，以及在NFCR工作人员讲授的年度流式细胞仪课程中用于讲授仪器原理的演示流式细胞仪。所有DiDAC系统都运行在LINUX 6.1操作系统下的Intel平台上。该系统具有革命性的能力，可以非常快速和复杂地控制我们所有细胞仪的操作，以及原始数据脉冲的四向排序和波形记录。DiDAC 1的设计已经冻结，新一代数据采集系统的设计已经开始。DiDAC I使用了大约6年前的技术，产生了物理上复杂的模块。商业流式细胞仪制造商对DiDAC I的一个反对意见是，每个检测器都需要昂贵的大画幅板。借鉴在DiDAC I开发中获得的经验，第二代系统的设计正在顺利进行。原型电路板将在资源年度结束前制造出来。DiDAC II是围绕具有可堆叠总线格式的商用数字信号处理器板构建的。NFCR设计的包含模数转换器和相关电路的电路板将直接插入堆栈。四个探测器的电路板将适合4 x 6 x 6英寸的体积。使用可编程DSP处理器可以通过软件实现高度的信号处理灵活性，这在DiDAC I中是不可用的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The DiDAC system is now in routine use on four LANL flow cytometers (two phase sensitive flow cytometers, the three-laser system and the rapid mix kinetics cytometer) as well as on the demonstration flow cytometer used to teach a lab on instrumentation principles during the Annual Course in flow cytometry taught in part by the NFCR staff. All the DiDAC systems are running on the Intel platform under the LINUX 6.1 operating system. This system has revolutionary capabilities for very fast and sophisticated control of the operation of all of our cytometers, as well as four-way sorting and waveform recording of the raw data pulses. The design of DiDAC I has been frozen and design of a new generation data acquisition system has begun. DiDAC I uses technologies that are ~6 years old resulting in physically complex modules. One objection of commercial flow cytometer manufacturers to DiDAC I has been that each detector requires an expensive, large format board. Drawing on the experience gained in the DiDAC I development, design of a second-generation system is well underway. The prototype circuit boards will be fabricated by the end of the Resource year. DiDAC II is built around a commercial digital signal processor board that has a stackable bus format. The NFCR designed boards that contain the analog to digital converters and associated circuitry will plug directly into the stack. Boards for four detectors will fit in a 4 x 6 x 6 inch volume. Use of a programmable DSP processor enables a high degree of flexibility in signal processing via software that is not available in DiDAC I.