X-RAY STUDIED OF REL-MTB

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。我们已经结晶Relmtb，一个来自结核分枝杆菌的Rel/SPoT同源物 调节细菌的严格反应。这个的淘汰赛 基因显示对抗生素的敏感性增加。我们已经收集了2.3 Ang分辨率的原生数据。但由于规模小（<50 这些数据的总体I/sigma（I）较低（~5）， Rsym相当高（0.16）。我们希望同步加速器的数据 改善这些问题，也将使我们能够解决 由MAD或MIRAS构建。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have crystallized Relmtb, a Rel/SPoT homolog from M tuberculosis that modulates the stringent response in that bacterium. Knockouts of this gene demonstrate increased susceptibility to antibiotics. We have collected native data to 2.3 Ang resolution. However, due to the very small (<50 um) crystal size, overall I/sigma(I) for these data are low (~5) and Rsym is rather high (0.16). We expect that synchrotron data will ameliorate these problems, and will also allow us to solve the structure by MAD or MIRAS.