COMPARATIVE PROTEOMIC INVESTIGATION OF ARDS AND SYSTEMIC INFLAMMATORY INJURY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。急性呼吸窘迫综合征(ARDS)是重症监护医学中导致发病率和死亡率的重要原因之一。它可能是许多疾病的后遗症，如败血症、胃内容物吸入、肺炎或重大创伤。该综合征的特征是炎症反应，导致肺泡-毛细血管屏障破裂，导致液体和蛋白质从血液进入肺泡腔。炎症反应的确切机制仍不完全清楚。已经进行了大量的临床和实验试验，以提高对这种疾病状态的了解和评估可能的治疗方案。方法：将ARDS患者的肺水肿液样本与心源性肺水肿患者的对照样本进行比较。蛋白质经层析、过滤和电泳层析分离后，用质谱仪(MALDI-TOF-TOF，ESI)分析两组蛋白质组和翻译后修饰，以确定疾病的蛋白质标志物。最终，浓度变化将通过免疫分析进行进一步评估。结果：通过柱层析和过滤，肺水肿液样品可以从碎片中清除，一维SDS-PAGE可以分离出蛋白质。目前正在评估MALDI-TOF-TOF的结果。高压液相色谱和电喷雾化分析定于下周进行。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The acute respiratory distress syndrome (ARDS) is one of the most important causes for morbidity and mortality in intensive care medicine. It can be the sequel of numerous diseases like sepsis, aspiration of gastric contents, pneumonia or major trauma. The syndrome is characterized by an inflammatory reaction that leads to a breakdown of the alveolar-capillary barrier, resulting in an influx of fluid and proteins from the blood into the alveolar space. The exact mechanism of the inflammatory reaction is still incompletely understood. Numerous clinical and experimental trials have been made in order to improve the understanding and evaluate possible treatment options of this disease state. METHODS: The proposed study focuses on the evaluation of pulmonary edema fluid samples of patients with ARDS compared to control samples from patients with cardiogenic pulmonary edema. After protein separation by chromatography, filtration and electrophoresis, mass spectrometry (MALDI-TOF-TOF, ESI) will be used to analyze the proteome and posttranslational modifications of proteins in both groups in order to identify protein markers of disease. Eventually, concentration changes shall be further evaluated by immunoassays. RESULTS: Using column chromatography and filtration, the pulmonary edema fluid samples were cleaned from debris and proteins could be separated by 1D SDS-PAGE. The results of MALDI-TOF-TOF are currently being evaluated. Analysis by High Pressure Liquid Chromatography and ESI is scheduled for next week.