TWO-HYBRID ANALYSIS OF SIFA

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。SifA是一种沙门氏菌效应蛋白，它是形成沙门氏菌诱导的细丝所必需的，这些细丝被转移到包裹病原体的液泡膜上，是病原体维持所必需的。米勒实验室的初步数据显示，当Sifa在酵母中表达时，存在明显的亚细胞定位。这项合作的目标是识别可能与SifA结合的潜在酵母蛋白并控制其定位。反过来，这将有助于阐明SifA在酵母细胞中可能参与的功能途径，并最终用于在哺乳动物细胞中设计实验。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SifA is a Salmonella effector protein required for the formation of Salmonella-induced filaments that is translocated across the vacuolar membrane enclosing the pathogen and is required for its maintenance. Preliminary data from Miller lab revealed a distinct subcellular localization when SifA was expressed in yeast. The goal of this collaboration is to identify potential yeast proteins that might be binding to SifA and controlling its localization. In turn, this will help elucidate the functional pathway SifA might be involved in within the yeast cell and eventually, used for designing experiments in mammalian cells.