TOR COMPLEX IN DICTYOSTELIUM

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。TOR通路已被证明整合营养和生长因子信号来控制细胞生长。最近，TOR在控制细胞骨架中的作用似乎是进化保守的。我们和Devreotes研究小组利用遗传筛选方法鉴定了Dictyostelium TOR （Dd-TOR）信号、RIP3和Pianissimo的组成部分。我们注意到Dd-TOR复合物含有另一个分子，因为我们在纯化Pianissimo时看到了几个共免疫沉淀带。我们希望鉴定Dd-TOR信号复合体的新成分，以揭示Dd-TOR途径在趋化性中的调节和重要性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The TOR pathway has been shown to integrate nutrient- and growth factor-signaling to control cell growth. Recently, the role of TOR in controlling the cytoskeleton appears to be evolutionarily conserved. We and Devreotes¿¿s group have identified the components of Dictyostelium TOR (Dd-TOR) signaling, RIP3 and Pianissimo, using genetic screening. We have noticed that the Dd-TOR complex contains another molecule, as we saw several co-immunoprecipitated bands when we purified Pianissimo. We wish to identify novel components of the Dd-TOR signaling complex to uncover the regulation, importance of Dd-TOR pathway in chemotaxis.