HUNTINGTIN PROTEIN INTERACTIONS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。亨廷顿蛋白是基因IT 15的产物，包含67个外显子，长度约为3150个氨基酸。蛋白质中谷氨酰胺重复延伸的扩展与致命的常染色体显性神经退行性疾病亨廷顿氏病有关。 胞质亨廷顿蛋白与微管共定位，并与β-微管蛋白相互作用，证据表明，亨廷顿蛋白参与细胞内运输，并可能与许多蛋白质伴侣相互作用。 在这里，野生型和异常的天然折叠亨廷顿蛋白将用于酵母ORF的系统筛选。这些数据应该有助于阐明天然折叠蛋白质的性质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Huntingtin protein is the product of gene IT15, encompassing 67 exons and is approximately 3150 amino acids in length. An expansion of a repeated stretch of glutamines in the protein is linked to the fatal, autosomal dominant neurodegenerative disorder, Huntington¿¿s disease. Cytoplasmic huntingtin colocalizes with microtubules and interacts with ss-tubulin and evidence may suggests that huntingtin is involved in intracellular transport and may interact with many protein partners. Here, both wild type and aberrant natively folded huntingtin will be used in a systematic screen of yeast ORFs. These data should help elucidate the nature of the natively folded protein.