SEARCH FOR SEC4P EFFECTORS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。在酿酒酵母中，从trans-Golgi到质膜的运输需要小的Rab-type GTdR Sec 4p。据认为Sec 4p被募集到trans-Golgi的分泌囊泡中，随后促进它们转运到质膜。在那里，Sec 4p似乎参与了外囊的组装，外囊是一种膜束缚复合物。然而，这个GTSTOM如何实现这些功能在很大程度上是不清楚的。 GTP酶是所谓的分子开关，其在开启（GTP结合）和关闭（GDP结合）状态之间循环。它们被认为是通过结合特定的所谓效应蛋白来实现其功能的。然而，到目前为止，只有一个效应器是已知的Sec 4p，这是Sec 15 p，在质膜外囊复合物的成员。基于我们对Sec 4p介导的分泌的广泛研究，该蛋白质不是Sec 4p实现其功能的唯一效应物。因此，本研究的目的是鉴定Sec 4p效应子并确定其作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The transport from the trans-Golgi to the plasma membrane in Saccharomyces cerevisiae requires the small, Rab-type GTPase Sec4p. It is thought that Sec4p is recruited to secretory vesicles at the trans-Golgi and subsequently facilitates their transport to the plasma membrane. There, Sec4p appears to be involved in the assembly of the exocyst, a membrane tethering complex. However, how this GTPase achieves these functions is largely unclear. GTPases are so-called molecular switches, which cycle between an ¿¿on¿¿ (GTP-bound) and an ¿¿off¿¿ (GDP-bound) state. They are thought to accomplish their function by binding specific, so-called effector proteins in their ¿¿on¿¿ state. However, so far only one effector is known for Sec4p, which is Sec15p, a member of the exocyst complex at the plasma membrane. Based on our extensive studies of Sec4p-mediated secretion, this protein cannot be the only effector through which Sec4p achieves its functions. Therefore, the objective of this research is to identify Sec4p effectors and determine their role.