MOLECULAR DISSECTION OF THE C ELEGANS KINETOCHORE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们目前正与伊恩麦克劳德在约翰耶茨的实验室进行生化分析的C。线虫和人类动粒。使用各种不同的纯化策略，我们试图分离所有已知的C。线虫和人类动粒蛋白，并在此过程中确定新的相互作用蛋白。而C.虽然秀丽线虫并不以优良的生物化学系统而闻名，但纯化策略的组合使我们能够纯化许多动粒蛋白。我们计划继续这项分析，并最终希望在这些动粒蛋白中进一步鉴定磷酸化位点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are currently working with Ian MacLeod in John Yates's lab to conduct a biochemical analysis of the C. elegans and human kinetochores. Using a variety of different purification strategies, we are attempting to isolate all of the known C. elegans and human kinetochore proteins and in the process identify novel interacting proteins. While C. elegans is not renowned for being an excellent biochemical system, the combination of purification strategies has allowed us to purify a number of kinetochore proteins. We plan to continue this analysis and ultimately hope to additionally identify phosphorylation sites in these kinetochore proteins.