STRUCTURAL DETERMINATIONS OF PROLAMINES FROM RICE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。脯胺是在种子中发现的主要储存蛋白质类型之一，因此是谷物蛋白质的膳食来源。脯胺保留在内质网腔内，并组装成内胆包涵颗粒。它们富含脯氨酸和谷氨酰胺。储存蛋白作为植物胚胎发育的氮源，在人体营养中也起着重要作用。储存的蛋白质可能与食物过敏有关。一些植物中的脯氨酸与乳糜泻有关。虽然水稻的情况并非如此，但他们对这种有机体的彻底调查也是有必要的。从大米中提纯了几种脯胺，并确定了它们的结构。正在研究的一个方面是糖基化的可能发生。用高效液相色谱法对大米中的脯胺进行了分离，并用质谱法对两个主要组分进行了研究。在这两个组分中，一个主要成分可以通过MALDI TOF质谱，以及在三重四极杆质谱、qoof质谱和FT-ICR质谱上的串联纳米喷雾质谱进行表征和部分测序。FT-ICR MS的进一步分析也应该允许对次要成分进行表征。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Prolamines are one of the major types of storage proteins found in seeds and thus are a dietary source of protein from grains. The prolamines are retained in the ER lumen and are assembled into intracisternal inclusion granules. They are rich in proline and glutamine. The storage proteins serve as a nitrogen source for the developing plant embryo and also play a role in human nutrition. It is possible that the storage proteins may be implicated in food allergies. Prolamines in some plants are involved in Celiac Disease. While this is not the case in rice, their thorough investigation is also warranted in this organism. Several prolamines from rice have been purified and their structures are being determined. One aspect being investigated is the possibe occurrence of of glycosylation. The prolamines from rice were separated by HPLC, and the two major fractions have been investigated by mass spectrometry. In both fractions, a major component could be characterized and partally sequenced by MALDI TOF MS, and by tandem nanospray MS on triple quadrupole, QoTOF, and FT-ICR mass spectrometers. Further analysis by FT-ICR MS should also allow for the characterization of the minor components.