FAST 3D PEDIATRIC BRAIN MRSI

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。前言：儿科神经光谱学提供了有关正常和异常脑发育过程中代谢变化的独特信息。光谱成像可以通过提供拓扑信息来增加这一特征，但临床可行的方案需要快速扫描时间。因此，我们开发了一种6分钟的3D MRSI临床方案进行研究。临床目标是通过分析皮质脊髓束(CST)中的代谢物水平来了解运动障碍受试者的代谢差异。方法和讨论：在3T下使用压力激发结合基于螺旋的读出梯度来开发3D MRSI序列。用于3T神经应用的双频带频谱空间自旋回波脉冲被设计为仅激发1%的水信号，并在通过NAA、Cr和CHO共振的同时抑制脂质。使用8通道接收器线圈的数据采集提供了更高的信噪比。总体而言，数据收集使用以下MRSI参数：3T，TR/TE=1500/130ms，32×32×8矩阵，1cc空间分辨率，6：24分钟扫描时间。量化是通过测量对应于CST的体素并将其合并到LC模型拟合过程中来执行的。活体研究表明，信噪比超过成人的结果很好。代谢物值从CST开始记录，开始于半卵圆中心外侧的中央前回，结束于大脑脚区域。在此年龄范围内，NAA/CHO显示出CST代谢物比率的线性变化趋势。需要进一步收集数据来证实这一趋势。无论如何，使用光谱成像分析整个CST途径(和其他途径)的光谱的能力是独一无二的。确认：NIH RR 09784、NS40117、NS46432、RR01271

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Pediatric neurospectroscopy provides unique information regarding the metabolic variations during normal and abnormal brain development. Spectroscopic imaging can add to this feature by providing topologic information, but rapid scan times are needed for clinical feasible protocols. We have thus developed a 6 min 3D MRSI clinical protocol to study. The clinical goal is to understand the metabolic differences in subjects with motor dysfunction by analyzing metabolite levels in the corticospinal tract (CST). Methods and Discussion: A 3D MRSI sequence was developed using PRESS excitation in combination with a spiral based readout gradient for use at 3T. A dualband spectral spatial spin echo pulse for 3T neuro applications, designed to excite only 1% of the water signal and suppress lipids while passing the NAA, Cr, and Cho resonances was used. Data collection using a 8 channel receiver coil provided increased signal to noise ratio (SNR). Overall, the following MRSI parameters were used for data collection; 3T, TR/TE = 1500/130 ms, 32x32x8 matrix, 1cc spatial resolution, 6:24 min scan time. Quantification was performed by measuring voxels corresponding to the CST and incoporating this into the LCModel fitting procedure. In vivo studies demonstrated excellent results with SNRs exceeding that from adults. Metabolite values were recorded from the CST beginning at the precentral gyrus lateral to the centrum semiovale and ending at the cerebral peduncle region. NAA/Cho showed a linear trend in the change of metabolite ratios for the CST given this age range. Further data collection is needed to confirm this trend. Regardless, the ability to analyze spectra from the whole CST pathway (and other pathways) is unique using spectroscopic imaging. Acknowledgements: NIH RR 09784, NS40117, NS46432, RR01271