SPONTANEOUSLY BIOTINYLATED LENTIVIRAL VECTORS FOR ENVELOPE INDEPENDENT TARGETING OF INFECTION

作为BBSRC资助项目的结果，我们已经产生了一种新的基于293T的细胞系，专门用于生产代谢生物素标记的慢病毒载体。这些生物慢病毒载体已被证明能有效地与链霉亲和素顺磁性颗粒复合。这种组合产生了迄今为止最有效的慢病毒载体纯化和浓缩方法。我们希望利用这些新的生物素-慢病毒包装细胞的平台来说明它们对基因治疗界的价值。由于其生物素化的表面，这些病毒可以附着在特定的细胞结合蛋白上，这样它们就可以靶向疾病的特定部位。它们的独特性质也使我们能够增加对细胞衍生的辅助蛋白的了解，这些辅助蛋白也驻留在慢病毒表面。关于这些蛋白对慢病毒感染过程影响的信息可用于更有效地将基因治疗载体靶向特定感染部位。

As a result of a BBSRC funded project we have generated a new 293T based cell line specifically for the production of metabolically biotin labelled lentiviral vectors. These bio-lentiviral vectors have been demonstrated to efficiently complex with streptavidin paramagnetic particles. This combination has resulted in the most efficient purification and concentration method yet described for lentiviral vectors. We wish to use the platform of these new biotin-lentiviral packaging cells to exemplify their value to the gene therapy community. By virtue of their biotinylated surface these viruses can be attached to specific cell binding proteins so that they can be targeted to specific sites of disease. Their unique properties also allow us to increase our knowledge of the cell derived accessory proteins that are also resident on the lentiviral surface. Information on the influence of these proteins on the process of lentiviral infection can then be applied to more efficient targeting of gene therapy vectors to specific sites of infection.