Biotinylated Monoclonal Antibody Conjugated to Gadolinium as a Contrast Agent for Magnetic Resonance Imaging of Human Rectal Carcinoma

生物素化单克隆抗体与钆缀合作为人直肠癌磁共振成像的造影剂

• 批准号: 17591429
• 负责人: OTSUJI Eigo
• 金额: $ 2.24万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591429/
• 关键词: pancreatic cancer; MRI; contrast agent; monoclonal antibody; 造影剤; キメラ化A7Fab分画; カドリニウム

Local disease is the most frequent recurrence pattern of rectal carcinoma, and its prognosis is not good. To detect local recurrence as early as possible, we produced the monoclonal antibody A7-gadolinium (Mab A7-Gd) conjugate as a contrast agent for magnetic resonance imaging to distinguish between carcinoma and normal tissue.We examined the in vitro immunoreactivity of Mab A7 coupled to Gd by chelate, isothiocyanobenzyl EDTA and its in vivo distribution in nude mice with human colorectal carcinoma.Mab A7-Gd retained binding activities that were nearly identical to intact Mab A7. More radiolabelled Mab A7-Gd accumulated in the tumor than normal mouse IgG-Gd. Both Mab A7-Gd and normal mouse IgG-Gd disappeared from blood linearly over time. Accumulation levels in normal tissues decreased linearly over time but were lower than those in tumors.The accumulation of biotinylated chA7Fab in the blood and the kidneys decreased significantly after the injection of avidin.

局部病变是直肠癌最常见的复发方式，预后不佳。为了尽早发现局部复发，我们制备了单克隆抗体A7-钆（Mab A7-Gd）偶联物作为磁共振成像的对比剂用于区分癌和正常组织。我们检测了通过螯合物偶联到Gd的Mab A7的体外免疫反应性，异硫氰基苄基EDTA及其在荷人大肠癌裸鼠体内分布Gd保留了与完整Mab A7几乎相同的结合活性。放射性标记的Mab A7-Gd在肿瘤中比正常小鼠IgG-Gd积累更多。Mab A7-Gd和正常小鼠IgG-Gd均随时间线性地从血液中消失。在正常组织中的积累水平随时间呈线性下降，但低于肿瘤中的水平。注射抗生物素蛋白后，生物素化chA 7 Fab在血液和肾脏中的积累显著下降。

项目成果

Monoclonal Antibody conjugated to Gadolinium as a contrast agent for magnetic resonance imaging of human rectal carcinoma.

与钆缀合的单克隆抗体作为人类直肠癌磁共振成像的造影剂。

• 期刊: J Surg Oncol (In press)
• 作者: Kuriu Y;Otsuji E;他
• 通讯作者: 他

Monoclonal antibody A7-superparamagnetic iron oxide as contrast agent of MR imaging of rectal carcinoma.

• DOI: 10.1038/sj.bjc.6602668
• 发表时间: 2005-07-11
• 期刊: British journal of cancer
• 影响因子: 8.8

Monoclonal antibody A7 coupled to magnetic particles as a contrast enhancing agent for magnetic resonance imaging of human colorectal carcinoma

• DOI: 10.1007/s00262-005-0067-9
• 发表时间: 2006-06-01
• 期刊: CANCER IMMUNOLOGY IMMUNOTHERAPY
• 影响因子: 5.8
• 作者: Otsuji，E;Kuriu，Y;Yamagishi，H
• 通讯作者: Yamagishi，H