Large scale lentiviral vector production

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Lentiviral vectors (LV) are remarkable in their ability to insert their genetic payload into a target cell's genome. This affects apermanent genetic change in the target which is propagated through to its progeny. While there are several means ofgenetic modification, few are capable of permanent modification of target cells. Amongst all vectors for gene delivery, LVare unique in unparalleled efficiency, safety, lack of toxicity and ability to modify non-dividing target cells. They havetherefore come to be recognised as a key reagent required for the efficient development of the burgeoning cell therapy, aswell as gene therapy industries. However, although LV represent a well understood and robust technology, there is nomanufacturing methodology for very large-scale LV production. This is now an acknowledged bottle-neck both for clinicaltrials and for commercial exploitation of many cell and gene therapy products in current development. We propose toaddress this unmet need