THE USE OF URINARY PCR TEST TO DETECT REJECTION IN KIDNEY TRANSPLANT PATIENTS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。尿聚合酶链反应测试已经被开发出来，以帮助检测肾移植过程中的排斥反应。对移植受者的尿液样本进行分析，找出已知在排斥反应中表达的特定基因，即颗粒酶B和穿孔素。此外，患者接受移植肾的“规程”活组织检查，目的是显示PCR结果与肾脏病理之间的相关性，当其他检查呈阴性时，也可用于检测排斥反应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The urinary PCR test has been developed to help detect rejection in kidney transplant procedures. A urine sample from a transplant recipient is analyzed for specific genes that are known to be expressed during rejection, namely Granzyme B and Perforin. In addition, patients undergo "protocol" biopsies of the transplanted kidney which aims to show the correlation between the PCR results and the pathology of the kidney, and may also serve to detect rejection when other tests are negative.