Bioprocessing of genetically engineered filamentous phages to underpin new therapeutic and industrial applications

在过去的50年中获得的噬菌体（也称为噬菌体和细菌病毒）遗传学和生理学的广泛知识使其成为开发药物，诊断和纳米技术应用产品的有吸引力的选择。在战胜传染病和遗传病的战斗中，改良的抗生素显示出了希望。例如，已经表明，展示在丝状噬菌体上的HIV表位可以引发T细胞和B细胞应答，为针对影响全世界约600万人的疾病的疫苗设计提供了新的方法。在大肠杆菌中可以合成高滴度的修饰型β-内酰胺酶。大肠杆菌中，原则上通过易于应用于相当大规模的技术进行加工。直到最近重新兴起的兴趣，作为抗微生物剂，有很少的研究关注大规模处理，因为一些早期的研究30年前。由于这个原因，现代生物化学工程和分子生物学的概念还没有结合起来，以解决所涉及的问题。拟议的研究将审查这些问题。随着微生物对抗生素耐药性危机的继续发展，抗生素也可能具有抗生素作用。虽然这不是本研究的主要重点，但所提出的生物化学工程研究将对这一相关领域产生影响。

The extensive knowledge of phage (also known as bacteriophages and bacterial viruses) genetics and physiology acquired in the past 50 years makes them an attractive option for development of products for pharmaceutical, diagnostic and nanotechnological applications. Modified phages are showing promise in the battle to defeat infectious and genetic diseases. For example, it has been shown that HIV epitopes displayed on a filamentous phage can elicit T-cell and B-cell responses offering a novel approach for vaccine design for a disease that affects approximately 6 million world-wide. Modified phages can be synthesised in high titres in E. coli and in principle processed by techniques readily applicable to considerable scale-up. Until a recently resurgence of interest in phages as anti-microbial agents there has been little research concern with large-scale processing since a few early studies 30 years ago. For this reason modern biochemical engineering and molecular biology concepts have yet to be brought together to address the issues involved. The proposed study will examine these. As the crisis in antibiotic drug resistance by microorganisms continues to develop it is also possible that phages will have an antibiotic role. Though this is not the primary focus of this research, the biochemical engineering studies proposed will bear on to this related field.