High Throughput Fractionation and Concentration System for MALDI-MS

描述(申请人提供)：用于MALDI-MS的高通量分离和浓缩系统这个小型企业创新研究(SBIR)第二阶段项目旨在以第一阶段可行性研究中完成的成功工作为基础，优化和构建一个并行制备多达96个血清样本的原型系统，用于基质辅助激光解吸电离(MALDI)质谱仪(MS)。该系统名为MALDIplex(Tm)M5样品准备站，由一个台式仪器、一个图形用户界面(GUI)和一次性使用的96孔盒组成。MALDIplex滤芯经过特殊设计，可通过一系列堆叠的薄层支架与每口井进行平行垂直分离。该系统允许集成样品净化、分馏、浓缩和除盐-所有这些都在一个易于使用的色谱盒中完成。使用该系统可以在不到45分钟的时间内同时制备样品。没有其他系统能够提供该系统所能达到的潜在吞吐量和数据质量。第二阶段的工作将侧重于进一步开发和优化定量和分馏方案，以及改进系统设计(用于自动化)和从墨盒表面识别蛋白质的方法。这项创新技术有可能在提高对癌症等复杂疾病的理解、诊断、预后和治疗方面发挥关键作用。如果成功，第二阶段将产生一个优化的样品制备系统，为第三阶段的全面生产和商业化做好准备。7.用于MALDI-MS的叙述性高通量分离和浓缩系统利用质谱学搜索新的药物靶标和疾病标记物(生物标记物)在改善对疾病的理解、诊断、预后和治疗方面具有巨大的潜力。许多复杂的、危及生命的疾病，如癌症，需要及早诊断，以最大限度地发挥有效治疗的作用。如果成功，这个第二阶段的研究项目将使用质谱学发现大规模的、与临床相关的生物标记物，这将有可能揭示用于早期疾病诊断和预测治疗反应的新的、高度敏感的生物标记物。

DESCRIPTION (provided by applicant): High Throughput Fractionation and Concentration System for MALDI-MS This Small Business Innovative Research (SBIR) Phase II project aims to build upon the successful work completed in the Phase I feasibility study to optimize and construct a prototype system for the preparation of up to 96 serum samples in parallel for matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS). This system, called the MALDIplex(tm) M5 Sample Prep Station, consists of a benchtop instrument, a graphical user interface (GUI), and single-use 96-well cartridges. The MALDIplex cartridges are specially designed to permit parallel vertical separation from each well through a series of stacked thin layer supports. The system permits integrated sample purification, fractionation, concentration, and desalting - all in a single, easy to use cartridge. Using this system, samples may be prepared simulaneously in less than 45 minutes. No other system offers the potential throughput and data quality achievable with this system. Phase II efforts will focus on the further development and optimization of quantitation and fractionation protocols, as well as improved system designs (for automation) and methods for protein identification from the cartridge surface. This innovative technology has the potential to play a key role in improving the understanding, diagnosis, prognosis, and treatment of complex disease such as cancer. If successful, the Phase II will result in an optimized sample preparation system ready for full scale manufacturing and commercialization in Phase III. 7. Project Narrative High Throughput Fractionation and Concentration System for MALDI-MS The search of novel drug targets and disease markers (biomarkers) using mass spectrometry holds tremendous potential for improving the understanding, diagnosis, prognosis, and treatment of disease. Many complex, life threatening diseases, such as cancer, require early diagnosis to maximize effective treatment. If successful, this Phase II research project will enable large scale, clinically-relevant biomarker discovery using mass spectrometry, which has the potential of revealing new, highly sensitive biomarkers for early disease diagnosis and to predict treatment response.