PROTEOMIC CHARACTERIZATION OF CEREBROSPINAL FLUID BY HIGH RESOLUTION LC-MS/MS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。迄今为止，该NCRR合作项目的重点是人类脑脊液（CSF）的蛋白质组学特征。在临床医学中，脑脊液是常规取样，以帮助确定和监测大脑或脊髓疾病的存在。临床医生通常为此目的测量总蛋白浓度，因为对正常或疾病状态下脑脊液的蛋白质组成或蛋白质组知之甚少。目前关于脑脊液的大部分知识都是基于二维凝胶分析技术，这种技术在灵敏度和通量方面都受到严重限制。本研究提出的研究总体目标有两个：(1)尽可能确定“正常”人脑脊液中蛋白质的含量和分布，为未来定量LC-FTICR研究创建准确的质量和时间标签数据库；(2)评估脑脊液中蛋白质的含量和丰度在脑和脊髓的特定疾病中是如何改变的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This NCRR collaborative project to date has focused on the proteomic characterization of human cerebrospinal fluid (CSF). CSF is routinely sampled in clinical medicine to help determine and monitor the presence of illness in the brain or spinal cord. Clinicians usually measure the total protein concentration for this purpose since very little is known about the protein composition, or proteome, of CSF in normal or disease states. Much of the current knowledge about CSF is based upon 2D gel analysis techniques which are severely limited in both sensitivity and throughput. The overall goals of the research proposed in this study are twofold: (1) determine, as possible, the content and distribution of proteins in "normal" human CSF for the creation of an accurate mass and time tag database for future quantitative LC-FTICR studies; and (2) Evaluate how the content and abundance of proteins in CSF are altered in specific diseases of the brain and spinal cord.