IDENT OF POST-TRANS MOD & PROTEIN COMPLEXES UNDER CONDITIONS OF OXIDATIVE STRES

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们的目标是了解观察到的钙稳态变化和细胞内蛋白质的氧化和硝化的机制，发生在一系列生物病理学，包括生物老化。在这项工作中，我们将集中在小鼠巨噬细胞的应激反应信号通路，涉及关键的钙调节蛋白钙调素（CaM）。我们可用的质谱分析工具将用于1）识别CaM结合伴侣及其参与信号传导途径2）研究硝化酪氨酸在调节过程中的作用，使用CaM作为模型蛋白质和3）探索CaM作为生物传感器的用途，以区分氧化应激的类型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goal is to understand the mechanisms underlying observed alterations in calcium homeostasis and the oxidation and nitration of intracellular proteins that occur under a range of biological pathologies, including biological aging. In this work we will focus on stress-response signaling pathways in mouse macrophages involving the critical calcium regulatory protein calmodulin (CaM). The mass spectrometry tools available to us will be used to 1) identify CaM binding partners and their involvement in signaling pathways 2) investigate the role of nitrated tyrosine in the regulatory processes, using CaM as a model protein and 3) explore the use of CaM as a biosensor to differentiate between types of oxidative stress.