Toxicogenimics Laboratory Core

该实验室核心的目标是提供基础设施 以及项目1-4的核心专业知识，以实现其目标。伯克利超级基金的总体目标 基础研究计划(SBRP)是应用功能基因组学、蛋白质组学、转录组学和 更好地检测砷、汞、苯、多环芳烃的纳米技术， 三氯乙烯和其他超级基金在环境中的优先化学品；评估它们对 人类健康，特别是儿童等易感人群的健康； 并降低它们的毒性。项目1到4将使用功能基因组学、蛋白质组学和 转录学在他们的研究中。此外，项目1和3是流行病学研究，需要 精密的样品处理，以便这些技术可以应用。项目的成功1- 4在很大程度上取决于生物样本的有效处理和管理以及获取 以及在最新的“经济”技术方面的专业知识。因此，详细的收集和存储协议具有 将设计并提供核心设施，用于细胞遗传学、基因分型、基因表达和 项目1-4中提出的蛋白质组学分析。核心C的具体目标是：1)过程、维护 和储存生物样本和细胞系；2)提供细胞遗传学的设施和方法 分析；3)使用Affymetrix、Illumina和定制阵列提供基因表达谱分析工具 技术；4)提供使用各种质谱学进行蛋白质组分析的设施 技术；以及，5)通过以下方式提供分析遗传多态的设施和方法 使用ABI 7900序列检测系统的TaqMan和珠子阵列技术 Illumina珠台站台。已获得所有必要领域的专业知识，以及 核心D的计算生物学技能，应该允许成功应用“组学”技术 在伯克利SBRP的项目1-4中。

The goal of this laboratory core is to provide the infrastructure and core expertise for Projects 1-4 to achieve their goals. The overall goal of the Berkeley Superfund Basic Research Program (SBRP) is to apply functional genomics, proteomics, transcriptomics, and nanotechnology to better detect arsenic, mercury, benzene, polycyclic aromatic hydrocarbons, trichloroethylene, and other Superfund priority chemicals in the environment; evaluate their effects on human health, especially the health of susceptible populations such as children; and remediate their presence and reduce their toxicity. Projects 1 through 4 will use functional genomics, proteomics, and transcriptomics in their studies. Further, Projects 1 and 3 are epidemiological studies that require sophisticated sample processing so that these technologies can be applied. The success of Projects 1- 4 largely depends on the effective handling and management of biological samples, as well as access to and expertise in the latest "-omic" technologies. Thus, detailed collection and storage protocols have been designed and core facilities will be provided for the cytogenetic, genotyping, gene expression and proteomic analyses proposed in Projects 1-4. The specific aims of Core C are to: 1) Process, maintain and store biological samples and cell lines; 2) provide facilities and methodologies for cytogenetic analysis; 3) provide facilities for gene expression profiling using Affymetrix, Illumina, and custom array technologies; 4) provide facilities for proteomic analyses using various mass spectrometric technologies; and, 5) provide facilities and methodologies for the analysis of genetic polymorphisms by Taqman-based and bead array technologies using the ABI 7900 Sequence Detection System and Illumina Bead Station platforms. Expertise in all required areas has been attained that, along with the computational biology skills of Core D, should allow the successful application of "-omic" technologies in Projects 1-4 of the Berkeley SBRP.