Identification of Altered Glycan and Glycoproteins in Viral Induced Liver Cancer

描述(由申请者提供)：这项研究拨款将试图确定所有在肝细胞癌患者血清中岩藻糖化水平增加的血清糖蛋白。在文献中，糖基化的改变在癌症中是公认的。更重要的是，我们已经证明，与肝癌的发展相关的核心岩藻糖化水平增加。然而，发生这些变化的蛋白质在很大程度上是未知的。因此，我们建议在癌前状态和癌状态下评估血清中与所有血清糖蛋白相关的岩藻糖化量的变化。这项比较研究将使用一种技术，该技术将利用各种岩藻糖识别凝集素来提取特定的糖蛋白，并使用基于LC MS/MS的蛋白质组学方法来鉴定它们。随着癌症的发展而改变的糖蛋白将被进一步评估其作为病毒诱导的肝癌生物标记物的潜力。

DESCRIPTION (provided by applicant): This research grant will attempt to identify all the serum glycoproteins that have increased levels of fucosylation in the sera of patients with hepatocellular carcinoma (HCC). It is acknowledged in the literature that changes in glycosylation occur with cancer. More importantly we have shown that there is an increase in the level of core fucosylation associated with the development of liver cancer. However, the proteins on which these changes occur are, for the most part, unknown. Hence, we propose to evaluate serum, in both a pre-cancerous and cancerous state, for alterations in the amount of fucosylation associated with all serum glycoproteins. This comparative study will use a technology that will exploit a variety of fucose recognizing lectins to extract specific glycoproteins and identify them using LC MS/MS based proteomic methodologies. Glycoproteins that alter with the development of cancer will be evaluated further for their potential as biomarkers of virally induced liver cancer.