IRREVERSIBLE AIRWAY DISEASE IN ASTHMA: EXHALED BREATH

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项研究的首要目标是了解儿童哮喘的病理生理学和自然病史。一小部分儿童哮喘患者出现肺功能损害的证据，这种损害可能是由于不可逆转的呼吸道疾病导致的药物治疗产生的。中心假设是，患有不可逆呼吸道疾病的年轻人可以通过CT显示的结构和病理生理变化以及乙酰甲胆碱气道敏感性增加来与对照组哮喘患者区分，而不是基于诱导痰或EBC。这项描述性的先导性研究试图使用两种新的非侵入性技术呼气冷凝物(EBC)和诱导痰(IS)来研究哮喘的气道重塑和炎症。从40名哮喘成人和40名年龄匹配的对照组采集的样本将提供EBS和IC样本。这些样本将被分析以寻找呼吸道炎症和重塑的标志。此外，还将分析几个方法学问题，包括日变化、受试者内部重复性、哮喘受试者和正常受试者之间的受试者差异、最佳采样时间。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overarching goal of this research is to understand the pathophysiology and natural history of childhood asthma. A small portion of childhood asthmatics develop evidence of lung function impairment that is resistant to pharmacotherapy that may be due to irreversible airway disease. The central hypothesis is that young adults with irreversible airway disease may be differentiated from control asthmatics by structural and pathophysiological changes demonstrated by CT and increased methacholine airway sensitivity but not on the basis of induced sputum or EBC. This descriptive, pilot study seeks to employ two novel, non-invasive techniques exhaled breath condensates (EBC) and induced sputum (IS) to study airway remodeling and inflammation in asthma. Samples collected from 40 asthmatic adults and 40 age-matched controls will provide EBS and IC samples. The samples will be analyzed for markers of airway inflammation and remodeling. In addition several methodologic issues will be analyzed including diurnal variation, intra-subject reproducibility, between subject variance for asthmatic and normal subjects, optimal sampling time.