INTRARENAL OXYGENATION AND OXIDANT STRESS IN PATIENTS WITH CKD AND CAN

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这项为期两年的前瞻性研究使用了一种称为血氧水平依赖性磁共振成像（BOLD MRI）的非侵入性成像技术。我们计划确定（1）慢性肾脏疾病（CKD）和慢性移植物肾病（CAN）是否与肾脏氧合不良相关（2）氧合不良是否会导致CKD和CAN中氧化应激（OS）增加（3）氯沙坦（一种血管紧张素受体阻滞剂）是否具有肾脏抗氧化特性。本研究将包括一组健康受试者和两组患者（CKD和CAN）。受试者将安排两次访视（间隔24个月），在50 mg口服剂量氯沙坦之前和之后采集血液和尿液样本。个体还将在氯沙坦之前和之后进行肾脏的BOLD MRI测试。将检查尿液和血液样本，以测量肾功能和OS的各种标志物。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This two-year prospective study uses a noninvasive imaging technique called blood oxygenation level-dependent magnetic resonance imaging (BOLD MRl). We plan to determine (1) whether chronic kidney disease (CKD) and chronic allograft nephropathy (CAN) are associated with poor kidney oxygenation (2) whether poor oxygenation results in increased oxidant stress (OS) in CKD and CAN (3) whether losartan, an angiotensin receptor blocker, has anti-oxidant properties in the kidney. The study will include a group of healthy subjects and two groups of patients (CKD and CAN). Subjects will be scheduled for two visits (24 months apart), where blood and urine samples will be collected before and after a 50 mg oral dose of losartan. Individuals will also undergo BOLD MRI testing of their kidney before and after losartan. Urine and blood samples will be examined to measure kidney function and various markers of OS.