COMPARISON OF TRIGLYCERIDES ON PLASMA LEVELS OF BETAHYDROXYBUTERANE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项初步研究的目的是确定中链甘油三酯(MCT)在测量脂肪酸氧化方面是否比长链甘油三酯(LCT)更好。脂肪酸是一种能量来源，通常这些脂肪在β-氧化过程中被转化为线粒体中的乙酰辅酶A。这项评估是由其他研究人员使用脂肪耐量测试进行的，时间间隔为10至12小时。测定血清β-羟丁酸(BHB)水平，计算曲线下面积。该方法可用于检测健康受试者与家族性混合性高脂血症患者之间约15%~20%的显著差异。此外，女性和男性之间的差异也更大。这项研究的目的是比较摄入两种口服脂肪负荷期间的BHB曲线，这两种脂肪负荷包括长链甘油三酯(LCT)和中链甘油三酯(MCT)。LCT由12到20个碳原子的脂肪酸组成；MCT包含长度为8到12个碳原子的脂肪酸。如下所述，MCT的链长和其他性质的差异将使MCT比LCT在更大程度上经历β-氧化。净效应应该是，在摄入MCT期间，BHb的血浆水平将高于LCT。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this pilot study is to determine whether Medium Chain Triglycerides (MCT) are better than Long Chain Triglycerides (LCT) in measuring fatty acid oxidation. Fatty acids are a source of energy and generally these fats are converted to acetyl coenzyme A in the mitochondria in the process of beta-oxidation. This assessment was done by other investigators using a fat tolerance test carried out over a 10 to 12 hour interval. Levels of beta-hydroxybutyrate (BHB) were measured and the area under the curve was calculated. The method was useful in detecting significant differences of about 15 to 20% between healthy subjects and those with familial combined hyperlipidemia. Also, somewhat greater differences were found between women and men. The study objective is to compare BHB curves during intake of two oral fat loads that consist of either long-chain triglycerides (LCT) or medium-chain triglycerides (MCT). LCT consists of fatty acids that have 12 to 20 carbon atoms; MCT contain fatty acids that are 8 to 12 carbon atoms in length. The difference in chain length and other properties of MCT, as detailed below, will allow MCT to undergo beta-oxidation to a greater extent than LCT. The net effect should be that plasma levels of BHB will be higher during the ingestion of MCT than LCT.