THE COMPARISON OF PLACENTAL AND UMBILICAL NUCLEATED RED BLOOD CELL COUNT

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。本项目将试图量化胎盘和脐带血中某种类型的血细胞（有核红细胞; nRBC）的存在和数量。胎盘是母亲为胎儿提供氧气和营养的器官。这个器官在出生后不再被母亲或胎儿需要，通常被丢弃或送往病理学。脐带连接到这个器官，并将血液输送到胎儿。脐带在出生后也被丢弃。该项目将涉及收集100名在Harbor-UCLA医学中心（HUMC）分娩的母亲的胎盘和脐带血。分娩后，将采集脐带动脉和静脉标本，用于pH和血气分析以及nRBC计数。胎盘组织将由HUMC病理学部门（Dr. L. McPhaul）进行nRBC计数。在新生儿出生后的前30分钟内，将采集毛细血管足跟采血样本，以检测pH值、血气和nRBC。 我们将评估脐动脉血、新生儿毛细血管足跟血和胎盘nRBC计数的关系。我们将进一步评估脐带和新生儿pH值和碱过剩值与nRBC水平的相关性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project will attempt to quantify the presence and number of a certain type of blood cell (nucleated red blood cell; nRBC) in the placenta and umbilical cord blood. The placenta is an organ used by the mother for oxygenation and nutrition of the fetus. This organ is no longer needed by the mother or the fetus after birth and is generally discarded or sent to pathology. The umbilical cord is attached to this organ and carries blood to and from the fetus. The umbilical cord likewise is discarded after birth. This project will involve collection of the placenta and umbilical cord blood of 100 mothers who deliver at Harbor-UCLA Medical Center (HUMC). Following delivery, umbilical cord artery and vein specimens will be obtained for pH and blood gas analysis and nRBC count. Placental tissue will be examined by the HUMC Pathology Dept (Dr. L. McPhaul) for nRBC count. During the first 30 minutes of newborn life, a capillary heel stick blood sample will be obtained for pH, blood gas and nRBC. We will assess the relation of umbilical arterial blood, and newborn capillary heel blood and placental nRBC count. We will futher assess the correlation of umbilical and newborn pH and base excess values to the level of nRBC.