AQUAPORIN GENE EXPRESSION IN HUMAN FETAL MEMBRANE

人胎膜中水通道蛋白基因的表达

• 批准号: 7606210
• 负责人: Michael Glenn Ross
• 金额: $ 0.13万
• 依托单位: LUNDQUIST INSTITUTE FOR BIOMEDICAL INNOVATION AT HARBOR-UCLA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7606210
• 关键词: Amniotic Fluid; Cell membrane; Cesarean section; Chorion; Computer Retrieval of Information on Scientific Projects Database; Fetal Membranes; Fetal Movement; Freezing; Funding; Gene Expression; Grant; Growth and Development function; Homeostasis; Human; Institution; Life; Liquid substance; Mediating; Membrane; Molecular; Nitrogen; Oligohydramnios; Pathway interactions; Placenta; Play; Pregnancy; Purpose; RNA; Reagent; Regulation; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Role; Source; Technology; Tissues; Umbilical cord structure; United States National Institutes of Health; Water; absorption; amnion; fetal; trizol; water channel; water flow

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Amniotic fluid (AF) is an essential accompaniment of normal pregnancy, necessary for fetal movement, growth and development. Oligohydramnios (OH), or reduced AF volume, occurs in 8 to 38% of all pregnancies. The intramembranous pathway of AF fluid absorption (across the amniotic membrane) has recently been recognized as a critical regulatory path for AF resorption, contributing importantly to AF volume homeostasis. Yet the underlying molecular and cellular mechanisms for water absorption across the amniotic membranes remain unknown. Since the discovery of AQP water channels about a decade ago, AQPs are increasingly recognized to play an important role in water transport across cell membranes. IM resorption of AF (water flow across the amnion and perhaps chorion, to the fetal vasculature) is potentially mediated via AQP water channel(s). However, of the eleven identified mammalian AQPs, few have been examined in fetal membranes. We have demonstrated AQP8 expression in both human and ovine amnion, chorion and placenta. These results suggest that AQP8, or other water channels may provide a pathway for fluid transport across fetal membranes, contributing to AF volume homeostasis. The purpose of the project is to investigate the aquaporins gene expression in human fetal membrane to further determine which water channels contribute to the regulation of amniotic fluid resorption. Human placenta and fetal membranes from normal term pregnancy will be studied. Immediately upon cesarean section delivery, amnion, chorion, placenta, and umbilical cord will be dissected and frozen in liquid nitrogen. Tissues will be stored at -70C until further analysis. Total RNA will be isolated from these tissues using Trizol reagent (Life Technologies, Gaithersburg, MD) for RT-PCR. RT-PCR will be used to detect the aquaporins gene expression in these tissues. We will use the discarded human placenta and fetal membranes from the subject upon delivery. Nothing will be done to the subjects.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 羊水（AF）是正常妊娠的重要伴随物，是胎儿运动、生长和发育所必需的。 8% 至 38% 的妊娠发生羊水过少 (OH) 或 AF 量减少。 AF 液体吸收的膜内途径（穿过羊膜）最近被认为是 AF 吸收的关键调节途径，对 AF 容量稳态有重要贡献。然而，羊膜吸水的潜在分子和细胞机制仍然未知。 自从大约十年前发现 AQP 水通道以来，人们越来越认识到 AQP 在跨细胞膜水运输中发挥着重要作用。 AF 的 IM 吸收（水流穿过羊膜和可能的绒毛膜，到达胎儿脉管系统）可能是通过 AQP 水通道介导的。然而，在 11 种已鉴定的哺乳动物 AQP 中，只有少数在胎膜中进行了检查。我们已经证明 AQP8 在人和羊的羊膜、绒毛膜和胎盘中表达。这些结果表明 AQP8 或其他水通道可能提供穿过胎膜的液体运输途径，有助于 AF 容量稳态。该项目的目的是研究人胎膜中水通道蛋白基因的表达，以进一步确定哪些水通道有助于调节羊水吸收。将研究正常足月妊娠的人类胎盘和胎膜。剖腹产后，立即解剖羊膜、绒毛膜、胎盘和脐带并冷冻在液氮中。组织将储存在-70°C直至进一步分析。使用 Trizol 试剂（Life Technologies，Gaithersburg，MD）从这些组织中分离总 RNA 进行 RT-PCR。 RT-PCR将用于检测这些组织中水通道蛋白基因的表达。 我们将使用受试者分娩时丢弃的人胎盘和胎膜。不会对受试者做任何事情。