EFFECT OF INSULIN ON PROTEIN SYNTHESIS IN PUBERTAL CHILDREN

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。本研究的目的是确定胰岛素在维持1型糖尿病儿童蛋白质合成中的重要性。本研究假设蛋白质代谢对胰岛素的需求与碳水化合物代谢对胰岛素的需求不同，维持儿童血糖控制可能无法为与生长相关的蛋白质代谢的合成代谢反应提供足够的胰岛素。我们将通过研究胰岛素对青春期1型糖尿病儿童的合成代谢作用来阐明这一假说。将在不含胰岛素的吸收后状态下测定全身蛋白质代谢，然后在提供胰岛素、氨基酸和葡萄糖的情况下测定。将根据L-[1- 13 C]亮氨酸的动力学评估胰岛素对全身蛋白质代谢的急性效应。通过测定胰岛素水平与正常喂养期间所达到的水平相当的蛋白质平衡，我们将能够确定儿童是否有足够的蛋白质平衡来维持生长。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to determine the importance of insulin in maintaining protein synthesis in children with Type 1 Diabetes. It is the hypothesis of the study that the requirement of insulin for protein metabolism is different from the insulin requirement for carbohydrate metabolism and that maintaining children in glycemic control may not provide sufficient insulin for anabolic responses of protein metabolism associated with growth. We will address this hypothesis by examining the anabolic effect of insulin in pubertal children with type 1 diabetes. Whole-body protein metabolism will be determined in the post absorptive state without insulin and then with the provision of insulin, amino acids, and glucose. The acute effect of insulin on whole-body protein metabolism will be assessed from the kinetics of L-[1-13C] leucine. By determining the protein balance in the presence of insulin at levels comparable to those achieved during normal feeding, we will be able to ascertain whether the children are sufficient protein balance to maintain growth.