CONSENT TO PARTICIPATE AS A DONOR OF PERIPHERAL BLOOD MONONUCLEAR CELLS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。艾滋病相关：一些研究人员正在开发基于未修饰或基因修饰T细胞过继性转移的新疗法，以恢复或改善宿主对病毒感染和恶性肿瘤的免疫反应。T细胞在体外生长到足以调节宿主免疫的数量，需要在培养物中添加大量的饲养细胞，以提供营养和生长因子。外周血单个核细胞（PBMC）通过白细胞分离从正常供体中采集，然后经过γ射线照射以阻止细胞生长，已被确定为饲养细胞的良好来源，并且是开发特异性过继免疫疗法所需要的。该方案招募正常的志愿捐献者进行白细胞分离，以获得用于开发免疫疗法的PBMC。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. AIDS related: Novel therapies based on the adoptive transfer of unmodified or genetically modified T cells are being developed by several investigators to restore or improve host immune responses to viral infection and malignancy. The growth of T cells in vitro to numbers sufficient to modulate host immunity requires the addition of large numbers of feeder cells to the cultures to provide nutrients and growth factors. Peripheral blood mononuclear cells (PBMC) harvested from normal donors by leukapheresis and then subjected to gamma irradiation to prevent cell growth have been identified as an excellent source of feeder cells and are needed for the development of specific adoptive immunotherapy. This protocol enrolls normal volunteer donors for leukapheresis to obtain PBMC for use in developing immune-based therapies.