The Role of ALIX during Multivesicular Body Biogenesis

细胞生长和分裂的速度是由来自环境的信号控制的。其中最重要的是一种循环的小蛋白质，叫做生长因子。它们与位于细胞外表面（或膜）的受体结合，导致它们改变与细胞内控制细胞生长的分子相互作用的模式。因此，生长因子受体是连接细胞内外的重要桥梁。为了防止反应继续以不受控制的方式进行，必须在生长因子结合后不久将受体从细胞表面移除，并将其送到称为溶酶体的专门细胞室，在那里它们被降解。受体首先从细胞表面移动到称为核内体的内部膜封闭腔室。在这里，受体被集中到膜的区域，这些区域向内进入内核体的内容物空间，然后被引导到溶酶体。确切地说，向内萌芽是如何发生的尚不清楚。本提案旨在通过使用一系列细胞生物学和生化技术来剖析这一过程的分子机制。

The rate at which cells grow and divide is controlled by signals from their environment. Amongst the most important of these is in the form of circulating small proteins called growth factors. These bind to receptors located on the cell's outer surface (or membrane), causing them to alter their pattern of interactions with molecules inside the cell that control cell growth. Growth factor receptors therefore act as essential bridges between the cell exterior and interior. In order to prevent responses continuing in an uncontrolled fashion, receptors must be removed from the cell surface shortly after growth factor binding and sent to a specialised cellular compartment called the lysosome, where they are degraded. Receptors first move from the cell surface to an internal, membrane-enclosed compartment called the endosome. Here the receptor is concentrated into regions of membrane that bud inwardly into the content space of the endosome, and are then directed to the lysosome. Precisely how inward budding occurs is not known. This proposal aims to dissect the molecular mechanisms underlying this process by using a range of cell biological and biochemical techniques.