THE EFFECT OF ISOFLURANE ON VOLUME DISTRIBUTION OF CRYSTALLOID SOLUTIONS IN

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在手术中，液体被注入血液中，以补偿手术中的液体损失和失血。手术创伤本身被证明会导致液体从血管中渗漏并随后积聚水肿物。麻醉剂还直接或间接地引起血管、心脏和肾脏功能的改变。全身麻醉期间的机械通气改变了血流动力学和液体需求。目前的科学观点认为，这种液体滞留和组织水肿是由手术创伤或机械通气引起的，而不是由麻醉剂本身造成的。然而，我们的研究小组最近在绵羊身上进行了两项研究，证明异氟醚麻醉和机械通风的结合显著改变了麻醉期间输液的处置。假设：我们假设在人类身上也会发现同样的结果。具体目标和程序：这个项目的目标是确定在人类静脉注射液体后，异氟醚是否减少尿量并促进外周液体积累，类似于早先在绵羊身上的发现。为了实现这一目标，我们将给麻醉的志愿者输液，并经常采集血红蛋白浓度的样本，血红蛋白浓度随着血浆容量的变化而变化。意义：如果被证实，这将是第一次被证明可以改变液体分布，从而促进人类组织的浮肿。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During surgery, fluids are given into the blood to compensate for fluid losses and blood loss during surgery. Surgical trauma itself is shown to cause fluid leakage from the vessels and subsequent accumulation of edema. Anesthetic agents also, directly or indirectly, cause changes in blood vessel, heart and kidney functions. Mechanical ventilation during general anesthesia alters hemodynamics and fluid requirements. The current scientific view is that this fluid retention and tissue edema are caused by surgical trauma or mechanical ventilation and not by the administration of an anesthetic per se. However, our research group has conducted two recent studies in sheep that have demonstrated that the combination of isoflurane anesthesia and mechanical ventilation significantly alters the disposition of an infused fluid during anesthesia. Hypothesis: We hypothesize that the same results will be found in humans. Specific Aims and Procedures: 'The goal of this project is to determine whether isoflurane reduces urinary output and promotes peripheral fluid accumulation after a bolus infusion of intravenous fluid in humans similar to findings earlier described in sheep. To accomplish this objective, we will give a fluid infusion to anesthetized volunteers and frequently sample hemoglobin concentration, which changes in concentration in response to changes in plasma volume. Significance: If corraborated, this would be the first time an anesthetic agent has been proven to alter fluid distribution and thereby promote tissue edema in humans.