FREE RADICALS, MEMBRANES AND ENZYME PHOTOACTIVATION

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。动脉斑块的形成是由于脂蛋白，特别是低密度脂蛋白的氧化。具体目的：本研究的目的是确定控制低密度脂蛋白（LDL）中脂质和蛋白质自由基氧化的机制。低密度脂蛋白是从新鲜的人血液中分离出来的，并暴露于游离药物引发剂中。分离了低密度脂蛋白氧化产物，并评价了天然和人造抗氧化剂的效果。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Development of arterial plaque results from oxidation of lipoproteins, particularly low-density lipoproteins. SPECIFIC AIM: The objective of this study is to determine the mechanisms governing the free radical oxidation of lipids and proteins in low-density lipoproteins (LDL). LDL are isolated from fresh human blood and exposed to free redical initiators. Products of LDL oxidation are isolated and the effect of natural and man-made antioxidants are assessed.