NE COBRE: MICROSCOPY CORE RESEARCH FACILITY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。UNL生物技术中心的显微镜核心研究设施积极参与了许多利用显微镜的研究，包括NCV成员和同事进行的研究。在2005 -2006财政年度（从2005年7月1日至2006年1月31日），显微镜核心已大量使用博士后研究人员，工作人员，研究生/本科生从实验室的NCV成员和联营公司（19名教师在本财年至今）。NCV成员对该核心的主要用途与免疫荧光共聚焦显微镜有关，用于病毒抗原的鉴定、定位和表征，并且一些用途与通过扫描或透射电子显微镜进行的超微结构和地形学研究有关，用于病毒颗粒/组分的可视化、鉴定、定位和表征。具有5个通道和6条激光线以及受控温度和CO2水平的倒置共聚焦为NCV成员和同事提供了用于活细胞成像的有用工具，并在本财年中得到了大量利用。此外，该核心还在倒置荧光显微镜上安装了高灵敏度CCD摄像头，供用户在不使用共聚焦系统的情况下采集荧光或传输图像。核心还帮助或参与了NCV成员今年提交的几项新的赠款提案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microscopy Core Research Facility of the Center for Biotechnology-UNL, has been actively involved in many studied utilizing microscopy, including those conducted by NCV members and associates. During FY2005-2006 (from July 1, 2005 to January 31, 2006), the Microscopy Core has been heavily utilized by postdoctoral researchers, staff, and graduate/undergraduate students from laboratories of the NCV members and associates (19 faculty members to date in this FY). The main usages of this core by NCV members are related to immunofluorescence confocal microscopy for identification, localization and characterization of viral antigens, and some uses are related to ultrastructural and topographic studies by scanning or transmission electron microscopy, for visualization, identification, localization and characterization of viral particles/component. A inverted confocal with 5 channels and 6 laser lines and controlled temperature and CO2 level, has provided NCV members and associates with a useful tool for live cell imaging and has been heavily utilized in this FY. In addition, the core has installed a high sensitivity CCD camera on an inverted fluorescence microscope for users to collect florescence or transmit images without using confocal system. The Core has also helped or participated in several new grant proposals submitted by NCV members in this year.