NMSU CELL AND ORGANISM CORE FACILITY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。新墨西哥州州立大学的细胞和有机体核心设施(COCF)提供了一个适当的设施，配备了仪器，用于利用新墨西哥州Brin/INBRE赠款的资金进行组织培养实验。对于NMSU生物和化学/生物化学系的初级教员在他们的研究中使用体外方法的研究项目来说，这个设施是一项重要的资产--一些初级教员也通过试点项目获得INBRE的资助。因此，COCF的功能符合新墨西哥州INBRE的声明目标之一：为研究人员和学生创建一个研究基础设施，以使用原代细胞培养物、来自各种动物生物体的活组织外植体制剂和已建立的细胞系进行研究。此外，COCF支持有利于NMSU非INBRE研究人员的研究活动，并促进社区中非大学科学家的使用。具体地说，COCF目前支持有学生参与的研究活动，重点是：1)脊椎动物再生过程中的细胞去分化和转分化(Graciela Unguez博士)，2)哺乳动物胚胎细胞中硬骨肌调节因子的功能特征及其成肌转换能力(Graciela Unguez博士)，3)涉及细胞质分裂和细胞周期调节的分子机制(Charles Shuster博士)，3)Grb7及其候选结合蛋白在哺乳动物细胞中的蛋白质-蛋白质相互作用(Barbara Lyons博士)，4)碳纳米管聚合物在哺乳动物细胞上的生物兼容性(Seamus Curran博士)，5)果蝇眼睛发育的机制(詹妮弗·柯蒂斯博士)，6)登革热病毒对其蚊子媒介的适应，以及这种适应如何影响病毒对人类的毒力(凯瑟琳·汉利博士)。COCF正在推动日常研究活动，不仅涉及教职员工，还涉及他们的研究生和本科生。在过去的8个月里，共有12名学生(8名研究生，4名本科生)接受了细胞培养技术的培训，并制定了针对组织特异性体外研究的优化方案。该设施的进一步成熟将允许更广泛的研究和教学项目，并支持更多的NMSU研究人员和访问科学家的需求。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell and Organism Core Facility (COCF) at New Mexico State University provides a proper facility equipped with instrumentation to carry out tissue culture experiments using funds from the New Mexico BRIN/INBRE grant. This facility is a critical asset to the research programs of junior faculty in the Biology and Chemistry/Biochemistry Departments at NMSU who use in vitro approaches in their studies - some junior faculty who also receive INBRE funding through pilot projects. Hence, the function of the COCF meets one of the stated goals of the New Mexico INBRE: to create a research infrastructure for investigators and students to perform studies using primary cell cultures, live tissue explant preparations from a variety of animal organisms, and established cell lines. In addition, the COCF supports research activities that benefit non-INBRE researchers at NMSU and promotes its use by non-university scientists in the community. Specifically, the COCF currently supports research activities that involve students and focus on: 1) cell dedifferentiation and transdifferentiation during regeneration in vertebrates (Dr. Graciela Unguez), 2) functional characterization of teleost muscle regulatory factors in mammalian embryonic cells and their myogenic conversion capacity (Dr. Graciela Unguez), 3) molecular mechanisms involved in the timing of cytokinesis and cell cycle regulation (Dr. Charles Shuster), 3) protein-protein interaction of Grb7 and its candidate binding proteins in mammalian cells (Dr. Barbara Lyons), 4) biocompatibility of carbon nanotube polymer on mammalian cells (Dr. Seamus Curran), 5) the mechanism of Drosophila eye development (Dr. Jennifer Curtis), and 6) the adaptation of dengue viruses to their mosquito vectors and how such adaptation affects virulence of the viruses for humans (Dr. Kathryn Hanley). The COCF is enabling daily research activities involving not only faculty, but their graduate and undergraduate students as well. In the past 8 months, a total of twelve students (8 graduate; 4 undergraduate) have been trained in cell culture techniques and have developed protocols optimized for tissue-specific in vitro studies. Further maturation of the facility will allow for a broader range of research and instructional projects and support the needs of additional NMSU researchers and visiting scientists.