NMSU CELL AND ORGANISM CORE FACILITY

NMSU 细胞和生物体核心设施

• 批准号: 7720449
• 负责人: Graciela Alexandra Unguez
• 金额: $ 9.46万
• 依托单位: NEW MEXICO STATE UNIVERSITY LAS CRUCES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720449
• 关键词: Animals; Binding Proteins; Biochemistry; Biology; Cell Culture Techniques; Cell Cycle Regulation; Cell physiology; Cells; Chemistry; Communities; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Cytokinesis; Daily; Faculty; Funding; Goals; Grant; In Vitro; Institution; Life; Mammalian Cell; Molecular; Muscle; Natural regeneration; New Mexico; Organism; Pilot Projects; Preparation; Primary Cell Cultures; Protocols documentation; Range; Research; Research Activity; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Scientist; Source; Students; Support of Research; Time; Tissues; Training; United States National Institutes of Health; Universities; Vertebrates; Visit; cell dedifferentiation; embryonic stem cell; established cell line; instrumentation; programs; protein protein interaction; research study; satellite cell; teleost; tissue culture; transdifferentiation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell and Organism Core Facility (COCF) at New Mexico State University provides a proper facility equipped with instrumentation to carry out tissue culture experiments using funds from the New Mexico BRIN/INBRE grant. This facility is a critical asset to the research programs of junior faculty in the Biology and Chemistry/Biochemistry Departments at NMSU who use in vitro approaches in their studies  and some junior faculty who also receive INBRE funding through pilot projects. Hence, the function of the COCF meets one of the stated goals of the New Mexico INBRE: to create a research infrastructure for investigators and students to perform studies using primary cell cultures, live tissue explant preparations from a variety of animal organisms, and established cell lines. In addition, the COCF supports research activities that benefit non-INBRE researchers at NMSU and promotes its use by non-university scientists in the community. The COCF currently supports research activities that involve students, staff and faculty and focus on 5 active research projects. These include: 1) molecular mechanisms involved in the timing of cytokinesis and cell cycle regulation (Dr. Charles Shuster) 2) cell dedifferentiation and transdifferentiation during regeneration in vertebrates (Dr. Graciela Unguez), 3) functional characterization of teleost muscle regulatory factors in mammalian embryonic stem cells and their myogenic conversion capacity (Dr. Graciela Unguez), 4) establishment of primary satellite cell culture from S. macrurus (Dr. Graciela Unguez), and 5) protein-protein interaction of Grb7 and its candidate binding proteins in mammalian cells (Dr. Barbara Lyons). The COCF is enabling daily research activities involving not only faculty, but their graduate and undergraduate students as well. In the past 10 months, a total of thirteen students (7 graduate, 6 undergraduate) have been trained in cell culture techniques and have developed protocols optimized for tissue-specific in vitro studies. Further maturation of the facility will allow for a broader range of research and instructional projects and support the needs of additional NMSU researchers and visiting scientists.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 新墨西哥州州立大学的细胞和生物体核心设施（COCF）提供了一个配备仪器的适当设施，利用新墨西哥州BRIN/INBRE资助的资金进行组织培养实验。 该设施是NMSU生物学和化学/生物化学系初级教师研究计划的重要资产，他们在研究中使用体外方法  还有一些初级教师也通过试点项目获得INBRE资助。 因此，COCF的功能符合新墨西哥州INBRE的既定目标之一：为研究人员和学生创建一个研究基础设施，以使用原代细胞培养物，各种动物有机体的活组织外植体制剂和已建立的细胞系进行研究。 此外，COCF支持有利于NMSU非INBRE研究人员的研究活动，并促进社区非大学科学家的使用。 COCF目前支持涉及学生，教职员工和教师的研究活动，并专注于5个活跃的研究项目。 其中包括：1）胞质分裂和细胞周期调控的分子机制（Dr. Charles Shuster）; 2）脊椎动物再生过程中的细胞去分化和转分化（Dr. Graciela Unguez）; 3）哺乳动物胚胎干细胞中硬骨鱼类肌肉调控因子的功能表征及其成肌转化能力（Dr. Graciela Unguez）; macrurus（Dr. Graciela Unguez），和5）哺乳动物细胞中Grb 7及其候选结合蛋白的蛋白-蛋白相互作用（Dr. Barbara里昂）。COCF使日常研究活动不仅涉及教师，但他们的研究生和本科生以及。 在过去的10个月里，共有13名学生（7名研究生，6名本科生）接受了细胞培养技术的培训，并开发了针对组织特异性体外研究优化的方案。 该设施的进一步成熟将允许更广泛的研究和教学项目，并支持更多NMSU研究人员和访问科学家的需求。