NMSU CELL AND ORGANISM CORE FACILITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 新墨西哥州州立大学的细胞和有机体核心设施（COCF）提供了一个设施，该设施配备了使用NM BRIN/INBRE赠款进行组织培养实验。 它是INBRE和非INBRE资助的初级教师的研究计划的重要资产，为研究人员和学生创建研究基础设施，使用原代细胞培养物，各种生物体的活组织外植体制剂和已建立的细胞系进行研究。COCF还促进社区中非大学科学家的使用。 COCF目前支持4个活跃的研究项目，涉及学生，教职员工和教师。 其中包括：1）胞质分裂和细胞周期调控的分子机制（Shuster）; 2）脊椎动物再生过程中的细胞去分化和转分化（Unguez）; macrurus（Unguez），和4）细胞内转染的新机制的开发（Smirnov）。COCF使日常研究活动不仅涉及教师，但他们的研究生和本科生以及。 在过去的12个月里，共有12名学生（6名研究生，6名本科生）接受了细胞培养技术的培训，这些学生开发了针对组织特异性体外研究优化的方案。 该设施的进一步成熟将包括更广泛的项目，并支持更多NMSU研究人员和访问科学家的需求。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Cell and Organism Core Facility (COCF) at New Mexico State University provides a facility equipped to carry out tissue culture experiments using funds from the NM BRIN/INBRE grant. It is a critical asset to the research programs of INBRE and non-INBRE funded junior faculty by creating a research infrastructure for investigators and students to perform studies using primary cell cultures, live tissue explant preparations from a variety of organisms, and established cell lines. The COCF also promotes its use by non-university scientists in the community. The COCF currently supports 4 active research projects that involve students, staff and faculty. These include: 1) molecular mechanisms involved in the timing of cytokinesis and cell cycle regulation (Shuster) 2) cell dedifferentiation and transdifferentiation during regeneration in vertebrates (Unguez), 3) establishment of primary satellite cell culture from a teleost vertebrate S. macrurus (Unguez), and 4) development of new mechanisms of intracellular transfection (Smirnov). The COCF is enabling daily research activities involving not only faculty, but their graduate and undergraduate students as well. In the past 12 months, a total of twelve students (6 graduate, 6 undergraduate) were trained in cell culture techniques and these students developed protocols optimized for tissue-specific in vitro studies. Further maturation of the facility will incorporate a broader range of projects and support the needs of additional NMSU researchers and visiting scientists.