SD COBRE: CELL IMAGING CORE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。为了让李发谦博士更专注于他的项目，Steven Armstrong博士最近被任命为该核心的主任。Armstrong博士在共聚焦显微镜方面拥有丰富的经验，他的办公室位于共聚焦室的正对面。Armstrong博士还带头为一种新的共聚焦显微镜提供了NIH设备补助金，该设备已于第4年重新提交。 拟议的项目1，2和3需要共聚焦显微镜。共聚焦显微镜更新了新软件。共焦系统的旧计算机现在是一个工作站，处理共焦系统采集的图像。我们广泛研究了G蛋白偶联受体激酶和黏着斑激酶在心肌肥厚和心力衰竭中的转位。已分析了用腺病毒构建体转染的培养心肌细胞中信号分子的细胞分布。我们建立了一个H-E染色站。我们还为PI提供冷冻切片。成像核心还为研究人员自己进行共聚焦显微镜检查提供技术培训和专业知识。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To allow Dr. Faqian Li to focus more on his project, Dr. Steven Armstrong was recently appointed Director of this core late in year 3. Dr. Armstrong has extensive experience in confocal microscopy and his office is located directly across the hall from the confocal room. Dr. Armstrong also spearheaded an NIH equipment grant for a new confocal microscope, which has been resubmitted in year 4. Proposed projects 1, 2, and 3 require confocal microscopy. The confocal microscope was updated with new software. The old computer for the confocal system is now a working station to process images acquired by the confocal system. We have extensively investigated the translocation of G-protein coupled receptor kinases and focal adhesion kinase during cardiac hypertrophy and failure. The cellular distribution of signaling molecules in cultured cardiac myocytes transfected with adenoviral constructs has been analyzed. We established an H-E staining station. We also provide frozen sectioning for PIs. The imaging core also provides technical training and expertise for investigators doing their own confocal microscopy.