TBI ELEVATIONS IN INTRACELLULAR CALCIUM CONCENTRATIONS BY NMDA RECEPTORS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。初步数据表明，GABA-A受体在实验性创伤性脑损伤（TBI）后发生改变（Gibson & Hamm, in review），然而，GABA-A受体亚基蛋白的改变尚未得到全面表征。目前的研究计划是评估哪些GABA-A受体亚基在一系列急性和慢性损伤后时间点被TBI改变。测定海马（选择性损伤易感区域）中GABA-A受体亚基的蛋白（1-2年）和mRNA（4-5年）表达。此外，拟议的钙介导级联将被药理学操纵（2-3年）。这个项目的时间表是如期完成的。正如INBRE赠款提案中最初指出的那样，项目的前9个月主要涉及设备和协议的设置。密集的数据收集计划于下个月开始。由于我的教学计划（主要精力分配在学年期间）与拟议的时间项目不相容，因此夏季的几个月（100%的拨款）将主要用于生成脑组织样本，然后在下一学年（2005-06）进行蛋白质分析。谈到我现在的INBRE学者的生产力，她今年花了很多时间来完成她的BRIN（生物医学研究基础设施网络）项目，她已经或即将向3个不同的听众做报告。我们都计划在秋天参加神经科学学会的会议。在第二年的资助期间，一位新入选的INBRE学者将开始与我一起开展上述INBRE项目。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Preliminary data indicate that the GABA-A receptor is altered following experimental traumatic brain injury (TBI) (Gibson & Hamm, in review), however, alterations to GABA-A receptor subunit proteins have yet to be comprehensively characterized. The current research plan is to evaluate which GABA-A receptor subunits are altered by TBI over a series of acute and chronic post-injury time points. Protein (years 1-2) and mRNA (years 4-5) expression of GABA-A receptor subunits will be measured in the hippocampus (an area selectively vulnerable to injury). Also, the proposed calcium-mediated cascade will be manipulated pharmacologically (years 2-3). The time line for this project is on target. As originally indicated in the INBRE grant proposal, the first nine months of the project have primarily involved equipment and protocol set-up. Intense data collection is planned to begin next month. Since my teaching schedule (primary effort allocation during the academic year) was incompatible with the timed projects proposed, the summer months (100% grant effort) will be primarily dedicated to generating brain tissue samples which can then be processed for protein analysis during the next academic year (2005-06). Addressing the productivity of my current INBRE scholar, she has devoted much of her time this year to completing her BRIN (Biomedical Research Infrastructure Network) project, which she has or will soon be presenting to 3 different audiences. We both plan to attend and present at the Society for Neuroscience convention in the fall. During the next (2nd) year of the grant a newly selected INBRE scholar will start working with me on the INBRE project described above.