RELEASE TIME TO STUDY THE PATHWAY OF 5'-NUCLEOTIDASE IN DICTYOSTELIUM

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。给出了释放时间，用于Dictyostelial5‘-Nuclotidase信号转导途径中基因的标记和特征研究。这将使研究开发与INBRE目前资助的微型赠款项目相一致。乌贝达特博士将设计几个实验和方法来标记作为5NT信号转导途径一部分的基因。要了解5‘-核苷酸酶基因及其蛋白产物的功能，需要对该基因信号转导途径中的各种突变体进行体内和体外分析。他的目标是确定这样的突变体，这些突变体可以用来识别基因正常运行所需的功能基因，以及细胞正常分化所必需的功能基因。使用REMI和其他方法，如微阵列和RNAi来分析基因表达，将提供更多关于网柄苔藓特定功能和作用的信息，可能也会提供更多信息，帮助理解其他生物如人类的功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Release time is given for the study of "Tagging and Characterization of Genes in the Signal Transduction Pathway of 5'-Nucloetidase in Dictyostelium." This will allow research development which will coincide with the mini-grant project currently being funded by INBRE. Dr. Ubeidat will design several experiments and approaches to tag genes that are part of the signal transduction pathway of 5nt. Understanding the function of 5'-nucleotidase gene and its protein product require both in vivo and in vitro analysis of various mutants in the signal transduction pathway of the gene. His goal is to identify such mutants that could be used to identify functional genes that are required for proper operation of the gene and necessary for the proper differentiation of the cells. Using REMI and other methods like microarray and RNAi to analyze gene expression will give more information about the specific function and role in Dictyostelium and probably more information to help understand the functions in other organisms like humans.